小花异裂菊根际与非根际微生物功能多样性比较  

Comparison of Microbial Functional Diversity Between Rhizosphere and Non-rhizosphere of Heteroplexis microcephala

作　　者：王博 覃芳 史艳财[3] 秦惠珍 邓丽丽 韦记青[3] WANG Bo;QIN Fang;SHI Yancai;QIN Huizhen;DENG Lili;WEI Jiqing(College of Tourism and Landscape Architecture,Guilin University of Technology,Guilin Guangxi 541006;College of Science,Guangxi Normal University,Guilin Guangxi 541006,China;Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guilin Guangxi 541006,China)

机构地区：[1]桂林理工大学旅游与风景园林学院,广西桂林541006 [2]广西师范大学生命科学学院,广西桂林541006 [3]广西壮族自治区中国科学院广西植物研究所,广西桂林541006

出　　处：《广西师范大学学报（自然科学版）》2022年第6期237-246,共10页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(31960276);中国科学院“西部之光”(2018);广西植物研究所基本业务费项目(桂植业19004)。

摘　　要：为阐明喀斯特石山特有植物小花异裂菊Heteroplexis microcephala Y.L.Chen.根际和非根际微生物代谢功能多样性及差异,揭示其生态适应机制,采用土壤养分分析常规方法和Biolog-ECO微平板法,对小花异裂菊5个典型分布地的根际和非根际土壤养分和微生物代谢功能多样性进行研究,结果表明:小花异裂菊根际土壤中,速效钾、全钾、pH、全氮、全磷、碱解氮、交换性钠以及交换性镁含量均高于相应的非根际土壤;根际土壤的微生物平均颜色变化率均高于非根际土壤;2个分布地根际土壤微生物多样性指数、优势度指数与非根际基本相同,其余3个分布地则根际明显高于非根际;根际与非根际土壤微生物主要利用的碳源类型均为羧酸和糖类化合物,4个分布地的根际微生物碳源利用率均明显高于非根际;主成分分析显示,糖类化合物是驱动小花异裂菊根际和非根际碳源代谢差异的主要碳源;冗余分析显示,全钾和交换性镁对小花异裂菊根际微生物的碳源利用模式影响呈正相关,全磷、速效磷、交换性钙与碳源利用呈负相关。本研究表明,通过分泌微生物代谢利用最广的糖类和羧酸类碳源来提高根际土壤微生物整体代谢活性、促进土壤养分循环并形成适宜自身的微生态环境是小花异裂菊适应喀斯特逆境的重要途径。To clarify the diversity and difference of microbial metabolic function between rhizosphere and non-rhizosphere,and be helpful for understanding the ecological adaptation of Heteroplexis microcephala and other endemic plants in karst area,five types of representative samples from Yangshuo County,Guangxi Zhuangzu Autonomous Region,were collected to analyze the microbial diversity and metabolic function by Biolog plate methods.The results showed that the values of pH and available K,total N,total P,total K,alkaline hydrolysis nitrogen,exchange calcium,and exchange magnesium content in the rhizosphere were higher than those in the non-rhizosphere.The average color change rate of microorganism in rhizosphere soil was higher than that in non-rhizosphere soil.The diversity index and dominance index of rhizosphere microorganisms in the two distribution sites were basically the same as those in the non-rhizosphere,while those in the other three distribution sites were significantly higher than those in the non-rhizosphere.The carbon sources used in rhizosphere and non-rhizosphere microbes were mainly sugars and carboxylic acids,and ability of utilize carbon source of four rhizosphere microorganisms were higher than those of non-rhizosphere.PCA results showed that sugars were the main carbon source driving the difference between those in rhizosphere and in non-rhizosphere.RAD results showed that differences in soil nutrient concentration did significantly impact the carbon source utilization of microorganisms in rhizosphere.Total potassium,exchange calcium and magnesiumand had a great impact on the carbon source utilization of microorganisms in rhizosphere.In general,the activity of the rhizosphere microorganisms and soil nutrient cycling were improved by secreting compounds such as carboxylic acid and sugar,which become the important way of beneficial micro ecological environment to adapting karst adversity.

关 键 词：小花异裂菊 根际土壤 土壤微生物 多样性 Biolog-ECO 

分 类 号：S793.9[农业科学—林木遗传育种]