非酒精性脂肪性肝病患者髓源性抑制细胞和白介素-6的初步研究  

Preliminary study on myeloid-derived suppressor cells and interleukin-6 in patients with non-alcoholic fatty liver disease

作　　者：庄静华 李明凯 陈式仪[3] 王贷明 ZHUANG Jinghua;LI Mingkai;CHEN Shiyi;WANG Daiming(Department of Rheumatology,Shantou Central Hospital,Shantou 515041,China;Department of Gastroenterology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510000,China;Department of Endocrinology and Rheumatology,the Second Affiliated Hospital of Shantou University Medical College,Shantou 515041,China;Department of Cardiology,the Second Affiliated Hospital of Shantou University Medical College,Shantou 515041,China)

机构地区：[1]汕头市中心医院风湿科,广东汕头515041 [2]中山大学第三附属医院消化内科,广东广州510000 [3]汕头大学医学院第二附属医院内分泌与风湿科,广东汕头515041 [4]汕头大学医学院第二附属医院心内科,广东汕头515041

出　　处：《汕头大学医学院学报》2022年第4期238-242,共5页Journal of Shantou University Medical College

基　　金：广东省医学科研基金(A2018432)。

摘　　要：目的:探讨非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)患者髓源性抑制细胞(myeloid-derived suppressor cells,MDSC)和白介素-6(interleukin-6,IL-6)的表达及意义。方法:选取2018—2019年在汕头大学医学院第二附属医院就诊的NAFLD患者30例(NAFLD组)和同期健康体检者30名(正常对照组)。采用流式细胞术检测外周血单个核细胞中MDSC及其亚型Mo-MDSC、G-MDSC的比例,采用酶联免疫吸附测定技术检测血清中IL-6表达水平,采用Spearman相关分析判断MDSC及其亚型、IL-6与丙氨酸转氨酶、天冬氨酸转氨酶等临床指标的关系。结果:NAFLD患者外周血中MDSC、Mo-MDSC、G-MDSC均明显低于正常对照组(P值均<0.05),IL-6明显高于正常对照组(P<0.05)。NAFLD患者外周血中G-MDSC的比例与丙氨酸转氨酶/天冬氨酸转氨酶呈负相关(r=-0.403,P=0.030)。NAFLD患者外周血中MDSC、Mo-MDSC、G-MDSC比例与IL-6均无相关性(P值均>0.05)。结论:MDSC、Mo-MDSC、G-MDSC、IL-6均可能参与了NAFLD的发生发展过程。Objective:To investigate the expression and significance of myeloid-derived suppressor cells(MDSC and interleukin-6(IL-6)in patients with non-alcoholic fatty liver disease(NAFLD).Methods:30 NAFLD patients(NAFLD group)and 30 healthy subjects(normal control group)who were treated in the Second Affiliated Hospital of Shantou University Medical College from 2018 to 2019 were selected.The proportion of MDSC and its subtypes Mo-MDSC and G-MDSC in peripheral blood mononuclear cells was detected by flow cytometry,and the expression level of IL-6 in serum was detected by enzyme-linked immunosorbent assay technology.Spearman correlation analysis was used to judge the relationship between MDSC and its subtypes,IL-6 and clinical indicators such as alanine aminotransferase and aspartate aminotransferase.Results:MDSC,Mo-MDSC,and G-MDSC in peripheral blood of NAFLD patients were significantly lower than those of healthy people(all P<0.05),and IL-6 was significantly higher than that of healthy people(P<0.05).The proportion of G-MDSC in peripheral blood of NAFLD patients was negatively correlated with the ratio of alanine aminotransferase to aspartate aminotransferase(r=-0.403,P=0.030).The proportions of MDSC,Mo-MDSC and G-MDSC in peripheral blood of NAFLD patients had no correlation with IL-6(all P>0.05).Conclusion:MDSC,Mo-MDSC,G-MDSC and IL-6 may all be involved in the occurrence and development of NAFLD.

关 键 词：非酒精性脂肪性肝病 髓源性抑制细胞 白介素-6 流式细胞术 

分 类 号：R575.5[医药卫生—消化系统]