核桃JrPUBs(U-box)基因家族鉴定与响应冷温胁迫基因筛选  

Identification of walnut JrPUBs(U-box)gene family and screening of genes in response to chilling stress

作　　者：姬新颖 冯启科 刘志月 刘凯 张俊佩[1] 王红霞[2] JI Xinying;FENG Qike;LIU Zhiyue;LIU Kai;ZHANG Junpei;WANG Hongxia(Research Institute of Forestry,Chinese Academy of Forestry/Key Laboratory of Tree Breeding and Cultivation of the State Forestry and Grassland Administration,Beijing 100091,China;Mountainous Areas Research Institute,Technology Innovation Center for Agriculture in Mountainous Areas of Hebei Province,Hebei Agricultural University,Baoding 071000,China;College of Horticulture,Hebei Agricultural University,Baoding071001,China)

机构地区：[1]中国林业科学研究院,林业研究所/国家林业和草原局林木培育重点实验室,北京100091 [2]河北农业大学,山区研究所/河北省山区农业技术创新中心/国家北方山区农业工程技术研究中心,河北保定071000 [3]河北农业大学园艺学院,河北保定071000

出　　处：《河北农业大学学报》2022年第6期68-80,共13页Journal of Hebei Agricultural University

基　　金：国家重点研发计划项目(2019YFD1001603);河北省重点研发计划项目(21326352D)。

摘　　要：为了解析核桃U-box基因家族在冷胁迫中的生物学功能,本文通过生物信息学在核桃全基因组中挖掘U-box(JrPUBs)成员,并对该家族成员进行分析,结合转录组数据筛选响应冷胁迫的差异表达基因,并分析该差异基因在不同组织中的转录水平差异。结果表明:在核桃(Juglans regia)全基因组中鉴定出55个JrPUBs基因,编码55条蛋白序列;聚类分析发现,核桃U-box家族成员分为2个亚组;冷胁迫下获得15个差异表达基因,其中JrPUB7、JrPUB9、JrPUB17、JrPUB18、JrPUB20、JrPUB21在根中转录水平较高;JrPUB42和JrPUB48在老叶中转录水平较高。推测该家族15个成员可能响应冷胁迫并参与植物器官发育的调控。In order to analyze the biological functions of the walnut U-box gene family,this study performed bioinformatics analysis to excavate U-box(JrPUBs)members in the walnut genome in response to chilling stress.The identified JrPUB members were analyzed on the physical and chemical properties,gene structure,conservative motifs,evolution relationship,cis-elements of the promoters and expressions.In the walnut(Juglans regia)genome,55 JrPUB members were identified encoding 55 protein sequences that clustered into 2 subgroups.According to the transcriptome data,15 JrPUB genes were differentially expressed in response to chilling stress and they were expressed in different tissues.The transcriptional levels of JrPUB7,JrPUB9,JrPUB17,JrPUB18,JrPUB20 and JrPUB21 were higher in roots.JrPUB42 and JrPUB48 were expressed higher in old leaves.JrPUB3,JrPUB9 and JrPUB12 were expressed lower in old leaves.Transcription of JrPUB42 was lower in tender stems and male flowers.It is speculated that 15 members of walnut U-box family may respond to chilling stress and participate in the regulation of plant organ development.

关 键 词：核桃 U-box基因家族 低温胁迫 基因筛选 

分 类 号：S664.1[农业科学—果树学]