伸筋草中1个新芒柄花萜醇衍生物  

A new onocerin-type triterpenoid derivative from Lycopodii Herba

作　　者：袁瑞瑛[1,2] 胡靖瑶 王希 谌顺清 蔡诗琪 王聪 蔡由生 YUAN Rui-ying;HU Jing-yao;WANG Xi;CHEN Shun-qing;CAI Shi-qi;WANG Cong;CAI You-sheng(Medical College,Tibet University,Lhasa 850012,China;School of Pharmaceutical Sciences,Wuhan University,Wuhan 430071,China;School of Chemistry and Chemical Engineering,Guangxi University for Nationalities,Nanning 530006,China)

机构地区：[1]西藏大学医学院,西藏拉萨850012 [2]武汉大学药学院,湖北武汉430071 [3]广西民族大学化学化工学院,广西南宁530006

出　　处：《中草药》2022年第21期6660-6665,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81973201);湖北省自然科学基金面上项目(2021CFB347)。

摘　　要：目的 研究伸筋草Lycopodii Herba的化学成分。方法 采用多种柱色谱对伸筋草95%乙醇提取物进行分离纯化;通过波谱数据对化合物进行结构鉴定;采用SRB法对所得化合物进行细胞毒性测试。结果 从伸筋草95%乙醇提取物中分离得到7个化合物,分别鉴定为3-甲酰基-芒柄花萜醇(1)、lycopodine(2)、lycoposerramine M(3)、4α-hydroxyanhydrolycodoline(4)、flabelliformine(5)、β-dihydrolycopodine(6)、lycodoline(7)。结论 化合物1为新的serratene型三萜,命名为3-甲酰基-芒柄花萜醇。活性测试结果表明化合物1没有明显的细胞毒活性,没有降糖活性。化合物5、6为首次从该植物中分离得到。Objective To study chemical constituents of Shenjincao(Lycopodii Herba). Methods Compounds in Lycopodii Herba were isolated and purified by column chromatography on silica gel column, Sephadex LH-20 gel column, and their structures were elucidated by analysis of its NMR and HRESIMS data. The cytotoxicity of the obtained compounds was evaluated by SRB methods.Results Seven compounds were obtained from 95% ethanol aqueous extract of Lycopodii Herba and their structures were identified as 3-formyloxyonocerin(1), lycopodine(2), lycoposerramine M(3), 4α-hydroxy-anhydrolycodoline(4), flabelliformine(5), β-dihydrolycopodine(6) and lycodoline(7). Conclusion Compound 1 was a new serratene-type triterpenoid named 3-formyloxy-onocerin, which showed no cytotoxicity, but could promote the glucose absorption of L6 cells. Compound 5 and 6 are isolated from this plant for the first time.

关 键 词：伸筋草 石松 serratene型五环三萜 3-甲酰基-芒柄花萜醇 细胞毒活性 flabelliformine β-dihydrolycopodine 

分 类 号：R284.1[医药卫生—中药学]