基于苦味阈值浓度的苦味中药分子苦度当量定量方法研究  

Study on quantitative method of equivalent molecular bitterness of traditional Chinese medicines based on bitterness threshold concentration

作　　者：王青晓[1] 高晓洁 桂新景 王艳丽[3,4,5] 王君明[4] 李晨旭 任延娜 姚静[3,4,5] 张璐[3,4,5] 施钧瀚[3,4,5] 李学林[3,4] 刘瑞新[3,4,5] WANG Qing-xiao;GAO Xiao-jie;GUI Xin-jing;WANG Yan-li;WANG Jun-ming;LI Chen-xu;REN Yan-na;YAO Jing;ZHANG Lu;SHI Jun-han;LI Xue-lin;LIU Rui-xin(Henan Institute for Food and Drug Control,Zhengzhou 450008,China;Department of Pharmaceutical Preparation,Traditional Chinese Medicine Hospital of Zhengzhou,Zhengzhou 450007,China;Department of Pharmacy,the First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450000,China;Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center of Chinese Medicine for Respiratory Diseases Co-construction by Henan Province&Ministry of Education,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南省食品药品检验所,河南郑州450008 [2]郑州市中医院制剂室,河南郑州1450007 [3]河南中医药大学第一附属医院药学部,河南郑州450000 [4]河南中医药大学,河南郑州450046 [5]河南中医药大学呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《中草药》2022年第21期6698-6705,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81774452);国家自然科学基金资助项目(81001646);2017年国家重点研发计划中医药现代化重点专项(2017YFC1703402);河南省中医药拔尖人才培养项目资助(2019ZYBJ07);河南省高层次人才特殊支持“中原千人计划”——“中原青年拔尖人才”项目(ZYQR201912158)。

摘　　要：目的建立适用于苦味中药的苦度定量方法。方法采用经典人群口感评价法,以盐酸小檗碱为参比苦味物质,以不同浓度的23种苦味中药饮片水煎液为载体,采用“最小极限法”预测中药饮片的苦味阈值浓度(bitterness threshold concentration,BTC),并在此基础上计算苦味中药的分子苦度当量(equivalent molecular bitterness,EMB)和分子苦度当量指数(EMB-index,EMBI),进而实现对中药饮片的苦度定量测定;此外,以不同浓度的黄连和苦参水煎液为载体,对建立方法的重现性进行验证。结果建立了感知到苦味的人数比例与苦味中药饮片水煎液浓度的对数模型和威布尔模型,由拟合方程的R~2和P值可知威布尔模型优于对数模型,由威布尔模型的拟合方程计算出中药饮片的BTC,进而测得了23种苦味中药饮片的EMB和EMBI;方法学验证结果显示,测得的黄连和苦参的EMB以及EMBI的RSD值均小于20%,重现性相对良好。结论采用经典人群口感评价法预测出了23种中药饮片的EMB和EMBI,建立了苦味中药的苦度定量测定方法。Objective To establish a quantitative method for bitterness intensity of bitter traditional Chinese medicines.Methods With the application of traditional human taste panel method(THTPM),the decoction of 23 kinds of bitter Chinese herbal pieces of different concentrations was used as the research subject and the“minimum limit method”was employed to predict the bitterness threshold concentration(BTC)of Chinese herbal pieces using berberine hydrochloride as the reference bitter substance.Based on this,the“equivalent molecular bitterness”(EMB)and molecular bitterness equivalent index(EMB-index,EMBI)of bitter Chinese medicine were calculated,so as to realize the quantitative determination of bitterness of Chinese medicinal slices.In addition,different concentrations of Huanglian(Coptidis Rhizoma)and Kushen(Sophorae Flavescentis Radix)decoction were used as carriers to verify the reproducibility of the established method.Results The logarithmic model and Weibull model of the proportion of the number of people who perceived bitter taste and the concentration of the decoction of bitter Chinese herbal pieces were established.From the R~2 and P value of the fitting equation,it can be seen that the Weibull model was better than the logarithmic model.The BTC of Chinese herbal pieces was calculated through the fitting equation yield by Weibull model,and then the EMB and EMBI of 23 kinds of bitter Chinese herbal pieces were measured;Methodological validation results showed that the RSD values of EMB and EMBI of Coptidis Rhizoma and Sophorae Flavescentis Radix were less than 20%,and the reproducibility was relatively well.Conclusion The EMB and EMBI of 23 kinds of Chinese herbal pieces was predicted through THTPM,and a quantitative determination method for the bitterness intensity of bitter traditional Chinese medicines has been established.

关 键 词：苦味中药 经典人群口感评价法 苦味阈值浓度 分子苦度当量 黄连 苦参 

分 类 号：R283.6[医药卫生—中药学]