3D打印PBS/CS房间隔缺损补片材料表征和细胞学测试  

CHARACTERIZATION AND CYTOLOGICAL TESTING OF 3D-PRINTED POLY (1,4-BUTYLENE SUCCINATE)/CHITOSAN ATRIAL SEPTAL DEFECT PATCH MATERIAL

作　　者：夏颖慧 邱水玮 孙江东 邢泉生[1] XIA Yinghui;QIU Shuiwei;SUN Jiangdong;XING Quansheng(Cardiac Center,Women and Children’s Hospital,Qingdao University,Qingdao 266034,China)

机构地区：[1]青岛大学附属妇女儿童医院心脏中心,山东青岛266034 [2]青岛大学医学部神经再生与康复研究院

出　　处：《青岛大学学报（医学版）》2022年第6期791-797,共7页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金资助项目(81570287)。

摘　　要：目的 探究聚丁二酸丁二醇酯(PBS)与壳聚糖(CS)复合材料3D打印制作房间隔缺损(ASD)补片的可行性及其细胞相容性。方法 通过密炼机共混出PBS/CS复合材料,应用傅立叶变换红外光谱仪和扫描电镜进行材料表征测试。通过3D热熔沉积打印评估不同比例PBS/CS材料的可打印性。phalloidin染色细胞骨架评估PBS/CS材料对小鼠胚胎成纤维细胞系NIH/3T3形态的影响,使用活性氧(ROS)检测试剂盒检测细胞内氧化应激水平,采用CCK-8法检测细胞增殖,应用Annexin V-FITC试剂盒进行凋亡程度检测。结果 材料表征测试显示PBS与CS成功混合,其中加入质量分数0.10 CS相较于加入质量分数0.20 CS混合更为均匀。3D打印出PBS/CS ASD补片,补片孔径一致,边缘光滑,对折后可恢复原状,但由于材料的结晶速度较慢,打印出的补片与理论尺寸存在一定的偏差。用PBS/CS材料浸提液培养的细胞与未用该材料浸提液培养的细胞比较,细胞形态、细胞内氧化应激水平以及细胞增殖和凋亡差异均无统计学意义。结论 3D打印PBS/CS共混材料制作ASD补片具有可行性,该复合材料对细胞增殖、凋亡、形态和氧化应激无明显影响。Objective To investigate the feasibility and cytocompatibility of the 3D-printed composite material of poly(1,4-butylene succinate)(PBS) and chitosan(CS) for fabricating atrial septal defect(ASD) patch. Methods PBS/CS compo-sites were blended in an internal mixer, and the material was characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. The 3D hot-melt deposition printing technique was used to evaluate the printability of materials with different PBS/CS ratios. Phalloidin staining of cytoskeleton was used to assess the influence of PBS/CS material on the morphology of mouse embryonic fibroblast cell line NIH/3T3;reactive oxygen species(ROS) kit was used to measure intracellular oxidative stress level;CCK-8 assay was used to measure cell proliferation, and Annexin V-FITC kit was used to measure the degree of apoptosis. Results Material characterization test showed that PBS and CS were successfully mixed, and the addition of CS at a mass fraction of 0.10 obtained a better mixture than the addition of CS at a mass fraction of 0.20. The 3D-printed PBS/CS ASD patch had uniform apertures and smooth edges and could be restored to its original shape after being folded. However, due to the slow crystallization velocity of the composite material, the printed patch had certain deviations from the theoretical size. There were no significant differences in cell morphology, intracellular oxidative stress level, cell proliferation, and cell apoptosis between the cells cultured in PBS/CS material extract and those not cultured in such extract. Conclusion It is feasible to use 3D-printed PBS/CS blending material to fabricate ASD patch, and the composite material has no significant influence on cell proliferation, apoptosis, morphology, and oxidative stress.

关 键 词：打印 三维 生物相容性材料 壳聚糖 房间隔缺损 

分 类 号：R318.08[医药卫生—生物医学工程] R541.4[医药卫生—基础医学]