猪肉可视化新鲜度智能指示薄膜研究  

Intelligent Indicator Film for Visual Meat Freshness Monitoring

作　　者：王玥[1,2] 樊柳荫 WANG Yue;FAN Liu-Yin(Student Innovation Center,Shanghai Jiaotong University,Shanghai 200240,China;School of Chemistry and Chemical Engineering,Shanghai Jiaotong University,Shanghai 200240,China)

机构地区：[1]上海交通大学学生创新中心,上海200240 [2]上海交通大学化学化工学院,上海200240

出　　处：《分析化学》2023年第1期139-145,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.22074091)资助。

摘　　要：基于静电纺丝技术,利用3种不同的pH敏感型指示剂分别与聚乙烯醇(PVA)共混,制备了3种可视化检测猪肉新鲜度的智能指示薄膜。采用扫描电子显微镜(SEM)对薄膜的微观结构进行了分析,测试了3种不同指示薄膜对氨气(NH3)及猪肉贮存过程中不同时段的颜色响应以及相应的色差值(ΔE)变化;采用自动凯氏定氮仪测定了不同贮存时间内猪肉的挥发性盐基氮(TVB-N)含量。结果表明,PVA/指示剂智能指示薄膜有较高的孔隙率与比表面积,对NH3的响应性良好,在猪肉贮藏过程中颜色变化明显,易通过肉眼观察。3种指示薄膜在猪肉不同贮存时间内的ΔE值与TVB-N含量均呈高度的相关性,可灵敏、准确地检测猪肉新鲜度变化,在肉类新鲜度的方便快捷、实时监测中具有良好的应用潜力。Three different pH-sensitive colorants including cyanidin cation(CC),bromothymol blue/methyl red(BTB/MR)and bromocresol purple(BCP)were respectively incorporated into polyvinyl alcohol(PVA)matrix to prepare intelligent indicator films by electrospinning for visual meat freshness monitoring.The morphology of films captured by scanning electron microscopy indicated that colorants had been successfully dispersed into PVA matrix and could be more sensitive due to the high specific surface area offered by the nanofiber structure.Each indicator film exhibited an obvious color response to volatile ammonia with a color difference value(ΔE)greater than the lowest distinguishable value(ΔE=7)when the mass fraction of colorant was increased to 3%.Color changes of intelligent indicator films during the minced pork freshness monitoring could be clearly observed by naked eyes and the correspondingΔE calculated according to the lightness(L),redness(a)and yellowness(b)of indicator films and total volatile basic nitrogen(TVB-N)contents determined by an automated Kjeldahl apparatus were positively correlated,which suggested that the intelligent indicator films could accurately indicate the freshness and held great promise in the application of rapid and real-time monitoring of meat freshness.

关 键 词：新鲜度 可视化 智能指示薄膜 聚乙烯醇 静电纺丝 

分 类 号：TS251.51[轻工技术与工程—农产品加工及贮藏工程]