帕米尔翠雀花的多糖成分及其活性研究  

Studies on the Polysaccharide from Delphinium lacostei Danguy and Its Biological Activity

作　　者：木尼热·阿布都克力木 周丽楠 郭远强[2] 木合塔尔·吐尔洪 Munira Abudukeremu;ZHOU Linan;GUO Yuanqiang;Muhetaer Tuerhong(College of Chemistry and Environmental Sciences,Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry,Kashgar University,Kashgar 844000;College of Pharmacy,Nankai University,Tianjin 300350)

机构地区：[1]喀什大学化学与环境科学学院,新疆喀什844006 [2]南开大学药学院,天津300350

出　　处：《分析科学学报》2022年第6期746-750,共5页Journal of Analytical Science

基　　金：新疆维吾尔自治区高校科研计划重大项目(XJEDU20171010);国家自然科学基金项目(21864016);新疆维吾尔自治区重点实验室开放课题(2017D04019)。

摘　　要：采用热水提取、乙醇沉淀、除蛋白等方法提取帕米尔翠雀花多糖成分,并对制备的帕米尔翠雀花多糖样品DL90的组成与含量进行了分析。根据红外光谱中的糖类化合物的特征峰,确认DL90主要含有糖类化合物。实验对DL90碳水化合物、蛋白质含量分别进行了测定,结果显示其含量分别为59.5%和4.6%。采用高效液相色谱分析对照品单糖组成,显示DL90是由甘露糖、鼠李糖、葡萄糖、半乳糖和阿拉伯糖5种单糖构成的杂多糖。为了探索帕米尔翠雀花的潜在应用价值,利用半叶枯斑法检测了DL90的抗烟草花叶病毒(TMV)活性。在体生物活性测试显示,在浓度为500 mg/mL时,DL90对感染TMV的保护作用、治疗作用和钝化活性,与阳性对照接近,表明DL90具有显著的抗TMV活性。该研究可为新疆高原植物帕米尔翠雀花的开发利用奠定基础。The polysaccharide from the plant Delphinium lacostei was prepared by hot water extraction and ethanol precipitation,etc.The characteristic peaks of sugars in the FT-IR spectrum confirmed that DL90 mainly contained sugars.The contents of carbohydrate and protein in the polysaccharide sample DL90 from D.lacostei were 59.5%and 4.6%respectively.Monosaccharide composition analysis showed that DL90 was composed of five monosaccharides.In vivo biological activity test showed that the protective effects,therapeutic effects,and passivation activity of DL90 on tobacco mosaic virus(TMV)infection were close to the positive control at the concentration of 500 mg/mL.In vivo virus activity test showed that DL90 had significant anti TMV activity.The studies on the plant D.lacostei lay a foundation for the development and utilization of the plateau plant D.lacostei in Xinjiang region.

关 键 词：帕米尔翠雀花 多糖 制备 抗病毒活性 

分 类 号：O657.72[理学—分析化学]