甲酸脱氢酶和甲醛脱氢酶异源表达及协同催化降解甲醛的研究  

Study on Heterologous Expression of Formate Dehydrogenase and Formaldehyde Dehydrogenase for Degradation of Formaldehyde

作　　者：莫易 彭念 陈雨点 李倩 刘文书 李勇昊 MO Yi;PENG Nian;CHEN Yu-dian;LI Qian;LIU Wen-shu;LI Yong-hao(School of Chemistry and Chemical Engineering,Chongqing University of Science and Technology,Chongqing Key Laboratory of Industrial Fermentation Microorganism(Chongqing University of Science and Technology),Chongqing 401331,China)

机构地区：[1]重庆科技学院化学化工学院,工业发酵微生物重庆市重点实验室(重庆科技学院),重庆401331

出　　处：《广州化工》2022年第22期62-64,共3页GuangZhou Chemical Industry

基　　金：重庆市自然科学基金项目(cstc2018jcyjAX0064);重庆科技学院硕士研究生创新计划项目(YKJCX2020539,YKJCX2120509,YKJCX2120523)。

摘　　要：利用生物酶降解建筑装修污染物甲醛具有温和及环保等特点而备受关注,但仅通过甲醛脱氢酶(FADH)催化甲醛为甲酸具有效率低及产生甲酸污染等系列问题。因此本研究采用大肠杆菌分别异源表达密码子优化的甲醛脱氢酶和甲酸脱氢酶基因。结果显示成功表达且纯化出具有活性的FADH和甲酸脱氢酶(FDH),进一步发现在FADH降解甲醛体系中加入FDH将甲酸进一步转化为无害的CO 2后甲醛降解效率可提高5.8倍,为“绿色”治理甲醛污染提供了可行性较高的方案。Bioenzymatic degradation of formaldehyde from architectural decoration pollution attracts high attention owing to the mildness and environmental friendliness of this route.However,catalysis of formaldehyde into formic acid by formaldehyde dehydrogenase(FADH)is restricted by inefficiency and formic acid pollution.Hence,Escherichia coli was applied into heterologous expression of codon-optimized genes in FADH and formate dehydrogenase(FDH),separately.Results showed that active FADH and active FDH were successfully expressed and purified.After FDH was added into the FADH-based formaldehyde degradation system,formic acid was further converted to harmless CO 2,which improved the formaldehyde degradation efficiency by 5.8 times.Hence,a highly-feasible scheme for“green”treatment of formaldehyde pollution was presented.

关 键 词：大肠杆菌 甲醛脱氢酶 甲酸脱氢酶 蛋白表达 甲醛 

分 类 号：X511[环境科学与工程—环境工程]