加压毛细管电色谱分离检测苦参中苦参碱成分  

Separation and Determination of Matrine in Sophora Flavescens by Pressurized Capillary Electrochromatography

作　　者：张潇晗 牛丽颖[1,2,3] 毕晓东 ZHANG Xiao-han;NIU Li-ying;BI Xiao-dong(School of Materia Medica,Hebei University of Chinese Medicine,Hebei Shijiazhuang 050091;Hebei Traditional Chinese Medicine Formula Granule Technology Innovation Center,Hebei Shijiazhuang 050091;Traditional Chinese Medicine Formula Granule Research Center,Hebei Province University,Hebei Shijiazhuang 050091,China)

机构地区：[1]河北中医学院,河北石家庄050091 [2]河北省中药配方颗粒创新中心,河北石家庄050091 [3]河北省中药材品质评价与标准化工程研究中心,河北石家庄050091

出　　处：《广州化工》2022年第22期133-134,152,共3页GuangZhou Chemical Industry

基　　金：河北中医学院科技能力提升项目(No:KTY2019067)。

摘　　要：建立了加压毛细管电色谱法(pCEC)分离苦参中苦参碱和氧化苦参碱两种生物碱类成分的方法。该方法采用EP-100-20/45-3-C18毛细管色谱柱(总长度45 cm,有效长度20 cm,直径为100μm,ODS填料3μm)进行分离,流动相为乙腈-3%乙酸溶液(97∶3),流动相的流速为0.06 mL/min,分离电压为0 kV,紫外检测波长为220 nm。结果表明,苦参碱和氧化苦参碱分离良好。该方法具有灵敏度高、操作简单、专属性强等优点,可同时检测苦参中苦参碱和氧化苦参碱,具有较大的应用价值。A method for the separation of matrine and oxymatrine from Sophora flavescens by pressurized capillary electrochromatography(pCEC)was established.Ep-100-20/45-3-c18 capillary column(total length 45 cm,effective length 20 cm,diameter 100μm,ODS packing 3μm)was used for separation.The mobile phase was acetonitrile-3%acetic acid solution(97∶3),the flow rate of mobile phase was 0.06 mL/min,the separation voltage was 0 kV,and the uv detection wavelength was 220 nm.The results showed that matrine and oxymatrine were separated well.This method has the advantages of high sensitivity,simple operation and specificity,and can simultaneously detect matrine and oxymatrine in Sophora flavescens.It has great application value.

关 键 词：加压毛细管电色谱 苦参 生物碱 

分 类 号：O652.7[理学—分析化学]