石草鞋化学成分及对人乳腺癌高转移细胞增殖的影响  

Chemical Constituents of Shicaoxie(Hoya Lyi Herba) and Their Effects on Cell Proliferation of Human Breast Cancer with High Metastasis

作　　者：唐素勤[1] 罗顺 TANG Suqin;LUO Shun(Affiliated Hospital of Henan Provincial Institute of Traditional Chinese Medicine,Zhengzhou 450004,China;Third Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450004,China)

机构地区：[1]河南省中医药研究院,河南郑州450004 [2]河南中医药大学第三附属医院,河南郑州450002

出　　处：《山东中医药大学学报》2023年第1期80-84,共5页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家中医药管理局全国中药特色技术传承人才培训项目(编号:T20184828005)。

摘　　要：目的:通过对石草鞋化学成分的分离及活性研究,揭示石草鞋抑制人乳腺癌高转移细胞增殖作用的物质基础。方法:对石草鞋90%乙醇提取物采用硅胶、葡聚糖凝胶LH-20(Sephadex LH-20)进行分离纯化,根据理化性质及波谱数据鉴定化合物结构;采用噻唑蓝(MTT)法测试化合物对人乳腺癌高转移细胞增殖的影响。结果:从石草鞋中分离得到12个化合物,分别鉴定为isoagatholal、monoeerin、对羟基肉桂酸甲酯、secologanoside、邻苯二甲酸二正丁酯、对羟基肉桂酸乙酯、isobonein、columbianetin acetate、gentioside、crototropone、3-羟基对甲氧基苯甲醛、2-desoxy-4-epi-pulchellin。monoeerin的半数抑制浓度(IC50)值为12.35μg/mL,crototropone的IC50值为9.51μg/m L。结论:所有化合物均为首次从石草鞋中分离得到,isoagatholal、monoeerin、isobonein、gentioside、crototropone为首次从该属植物中分离得到;monoeerin、crototropone可抑制人乳腺癌高转移细胞增殖。Objective:To reveal the material basis of inhibiting cell proliferation of human breast cancer with high metastasis by the separation and activity study on the chemical constituents of Shicaoxie(Hoya Lyi Herba). Methods:The 90% ethanol extract was isolated and purified by silica gel and Sephadex LH-20,and the structure of the compound was identified according to the physicochemical properties and spectral data. The effect of the compound on cell proliferation of human breast cancer with high metastasis was tested by MTT assay. Results:Twelve compounds were isolated from Shicaoxie and identified as isoagatholal, monoeerin, methyl para-hydroxycinnamate,secologanoside,din-butyl phthalate,ethyl para-hydroxycinnamate,isobonein,columbianetin acetate,gentioside,crototropone,3-hydroxy-methoxybenzaldehyde,2-desoxy-4-epi-pulchellin. The IC50value of monoeerin and crototropone was 12.35 μg/mL and 9.51 μg/mL respectively. Conclusions:All the compounds have been isolated from Shicaoxie for the first time. Isoagatholal,monoeerin,isobonein,gentioside and crototropone have been isolated from this genus for the first time. Monoeerin and crototropone can inhibit the proliferation of high metastatic cells in human breast cancer.

关 键 词：石草鞋 化学成分 分离鉴定 波谱数据 噻唑蓝法 人乳腺癌高转移细胞 细胞增殖 

分 类 号：R284.1[医药卫生—中药学]