miR-339对气道平滑肌细胞增殖及ERK1/2通路的影响  

Effects of miR-339 on the proliferation of airway smooth muscle cells and the ERK1/2 pathway

作　　者：沈文婷 秦建品 许诣 SHEN Wenting;QIN Jianpin;XU Yi(Department of Pediatrics,Xiangyang Central Hospital,Affiliated Hospital of Hubei University of Arts and Science,Xiangyang 441021,China)

机构地区：[1]湖北文理学院附属医院/襄阳市中心医院儿科,湖北襄阳441021

出　　处：《长春中医药大学学报》2023年第1期48-52,共5页Journal of Changchun University of Chinese Medicine

基　　金：湖北省自然科学基金项目(WJ2017Q039)。

摘　　要：目的对miR-339进行过表达后,探究其对气道平滑肌细胞(ASMCs)增殖及细胞外调节蛋白激酶1/2(ERK1/2)通路的影响。方法体外培养ASMCs细胞,设置对照组、模型组(5μg·L^(-1)TGF-β_(1))、miR-NC组(miR-NC+5μg·L^(-1)TGF-β_(1))、miR-339 mimics组(miR-339 mimics+5μg·L^(-1)TGF-β_(1))、miR-339 mimics+PD98059组(miR-339 mimics+10μmol·L^(-1)ERK1/2抑制剂PD98059+5μg·L^(-1)TGF-β_(1))。采用qRT-PCR检测各组miR-339的表达;MTT法检测ASMCs细胞活力;流式细胞术检测ASMCs细胞凋亡情况;Western blot检测ASMCs细胞内增殖、凋亡及通路相关蛋白表达情况。结果与对照组相比,模型组ASMCs细胞存活率、c-Myc、CyclinD1、PCNA、Bcl-2、p-ERK1/2/ERK1/2表达水平显著增加(P<0.05),细胞凋亡率、Caspase-3、Bax表达水平显著降低(P<0.05);miR-339 mimics组与模型组比较、miR-339 mimics+PD98059组与miR-339 mimics组比较,ASMCs细胞存活率、c-Myc、CyclinD1、PCNA、Bcl-2、p-ERK1/2/ERK1/2表达水平均显著降低(P<0.05),细胞凋亡率、Caspase-3、Bax表达水平均显著增加(P<0.05)。结论miR-339过表达对ASMCs细胞增殖的抑制,可能与抑制ERK1/2信号通路有关。Objective To explore the effects of an overexpression of miR-339 on the proliferation of airway smooth muscle cells(ASMCs)and the extracellular regulatory protein kinase 1/2(ERK1/2)pathway.Methodss ASMCs cells were cultured in vitro and divided into a control group,a model group(5μg·L^(-1)TGF-β_(1)),an miR-NC group(miR-NC+5μg·L^(-1)TGF-β_(1)),an miR-339 mimics group(miR-339 mimics+5μg·L^(-1)TGF-β_(1))and an miR-339 mimics+PD98059 group(miR-339 mimics+10μmol·L^(-1)ERK1/2 inhibitor PD98059+5μg·L^(-1)TGF-β_(1)).In each group,the expression of miR-339 was detected by qRT PCR,the cell viability of ASMCs was detected by MTT assay,the apoptosis of ASMCs was detected by flow cytometry,and the proliferation,apoptosis and pathway-related protein expression in ASMCs was detected by Western blot.Resultss Compared with the control group,the survival rate and the expression levels of c-Myc,CyclinD1,PCNA,Bcl-2,p-ERK1/2/ERK1/2 of ASMCs were significantly increased in the model group(P<0.05),while the apoptosis rate and the expression levels of Caspase-3 and Bax were significantly decreased(P<0.05).Compared with the model group and compared with the miR-339 mimics group,the ASMCs survival rate and the expression levels of c-Myc,CyclinD1,PCNA,Bcl-2,p-ERK1/2/ERK1/2 were significantly reduced(P<0.05),while the cell apoptosis rate and the expression levels of Caspase-3,Bax were significantly increased(P<0.05),respectively than those in the miR-339 mimics group and in the miR-339 mimics+PD98059 group.Conclusion The overexpression of miR-339 inhibits the proliferation of ASMCs,which may be related to its inhibition of ERK1/2 signaling pathway.

关 键 词：气道平滑肌细胞 微小RNA 339 细胞外调节蛋白激酶 增殖 

分 类 号：R563[医药卫生—呼吸系统]