猪链球菌2型HNB-LAMP检测方法的建立  

Development of HNB-LAMP to Detect Streptococcus Suis Serotype 2

作　　者：席小燕 陈家享 梁嘉雯 林蝶 伍嘉慧 段昌平 陈家苑 彭凌[2] XI Xiao-yan;CHEN Jia-xiang;LIANG Jia-wen;LIN Tie;WU Jia-hui;DUAN Chang-ping;CHEN Jia-yuan;PENG Ling(Medical College,Shaoguan University,Shaoguan 512026,Guangdong,China;Henry Fok School of Biology&Agriculture,Shaoguan University,Shaoguan 512005,Guangdong,China)

机构地区：[1]韶关学院医学院,广东韶关512026 [2]韶关学院英东生物与农业学院,广东韶关512005

出　　处：《韶关学院学报》2022年第12期50-54,共5页Journal of Shaoguan University

基　　金：韶关学院大学生创新创业训练计划项目(S202210576029);韶关市科技计划项目(210724094530192)。

摘　　要：根据GenBank公布的猪链球菌2型的cps2J基因的保守序列,采用在线引物设计软件设计LAMP引物,优化LAMP反应体系与反应条件,通过敏感性比较,优选出合适的扩增产物检测方法,最后对检测方法的特异性进行评估.实验表明HNB检测扩增产物敏感性高且方便使用,为合适的扩增产物检测的方法.建立的HNB-LAMP方法可以在50 min完成检测;与其它细菌无交叉反应;与普通PCR检测相比,敏感性提高了100倍.建立的HNB-LAMP检测系统简单、快速、敏感,在检测猪链球菌2型方面具有良好的临床潜力,适合基层单位使用.LAMP primers were designed by online primer design software according to the conserved sequence of cps2J gene of Streptococcus suis serotype 2 published in GenBank.The LAMP reaction system and reaction conditions were optimized.By comparing the sensitivity of detection,the appropriate detection method for LAMP amplification products was preferred,and the specificity of the LAMP method was evaluated finally.The results showed that HNB detection was a suitable method for detecting amplification products because of its high sensitivity and convenient use.Streptococcus suis serotype 2 could be identified in 50 min by the established HNB-LAMP method,without any cross reaction with other bacteria,and its sensitivity was 100 times higher than conventional PCR.The HNB-LAMP detection system established was simple,rapid and sensitive,and had good clinical potential in the detection of Streptococcus suis serotype 2,which is suitable for using in base unit.

关 键 词：猪链球菌2型 环介导等温扩增 检测 

分 类 号：R378.1[医药卫生—病原生物学]