Peripheral nerve regeneration through nerve conduits evokes differential expression of growth-associated protein-43 in the spinal cord  

作　　者：Jesús Chato-Astrain Olga Roda David Sánchez-Porras Esther Miralles Miguel Alaminos Fernando Campos Óscar Darío García-García Víctor Carriel 

机构地区：[1]Department of Histology(Tissue Engineering Group),University of Granada,Instituto de Investigación Biosanitaria ibs.GRANADA,Granada,Spain [2]Department of Human Anatomy and Embryology,University of Granada,Granada,Spain [3]Unit of Clinical Neurophysiology,University Hospital San Cecilio,Granada,Spain

出　　处：《Neural Regeneration Research》2023年第8期1852-1856,共5页中国神经再生研究（英文版）

基　　金：financed by the Spanish"Plan Nacional de Investigación Cientifica,Desorrollo e Innovación Tecnológica,Ministerio de Economíay Competitividod(Instituto de Solud CarlosⅢ)",grant Nos:FIS PI17-0393,FIS PI20-0318;co-financed by the"Fondo Europeo de Desorrollo Regional ERDF-FEDER European Union",grant No.P18-RT-5059;by"Plan Andaluz de Investigación,Desarrollo e Innovación(PAIDI 2020);Consejerio de Transformoción Económico,Industria,Conocimiento y Universidades,Junta de Andolucío,Espa?a",and grant No.A-CTS-498-UGR18;by"Programa Operotivo FEDER Andalucía 2014-2020,Universidod de Granada,Junta de Andalucía,Espa?a",ca-funded by ERDF-FEDER,the European Union(all to VC)。

摘　　要：Growth-associated protein 43 plays a key role in neurite outgrowth through cytoskeleton remodeling.We have previously demonstrated that structural damage of peripheral nerves induces growth-associated protein 43 upregulation to promote growth cone formation.Conversely,the limited regenerative capacity of the central nervous system due to an inhibitory environment prevents major changes in neurite outgrowth and should be presumably associated with low levels of growth-associated protein 43 expression.However,central alterations due to peripheral nerve damage have never been assessed using the growthassociated protein 43 marker.In this study,we used the tubulization technique to repair 1 cm-long nerve gaps in the rat nerve injury/repair model and detected growth-associated protein 43 expression in the peripheral and central nervous systems.First,histological analysis of the regeneration process confirmed an active regeneration process of the nerve gaps through the conduit from 10 days onwards.The growth-associated protein 43 expression profile varied across regions and follow-up times,from a localized expression to an abundant and consistent expression throughout the regeneration tissue,confirming the presence of an active nerve regeneration process.Second,spinal cord changes were also histologically assessed,and no apparent changes in the structural and cellular organization were observed using routine staining methods.Surprisingly,remarkable differences and local changes appeared in growth-associated protein 43 expression at the spinal cord level,in particular at 20 days post-repair and beyond.Growth-associated protein 43 protein was first localized in the gracile fasciculus and was homogeneously distributed in the left posterior cord.These findings differed from the growth-associated protein 43 pattern observed in the healthy control,which did not express growth-associated protein 43 at these levels.Our results revealed a differential expression in growth-associated protein 43 protein not only in the regeneratin

关 键 词：growth-associated protein 43(GAP-43) IMMUNOHISTOCHEMISTRY nerve guide nerve tissue regeneration peripheral nerve repair spinal cord tissue engineering 

分 类 号：R651.2[医药卫生—外科学]