烟草赤星病生防菌的分离与鉴定  

Isolation and Identification of Biocontrol Bacteria of Tobacco Brown Spot Disease

作　　者：郝浩浩 李翔 周权 吕浩秋 余立业 孙会忠[2] HAO Haohao;LI Xiang;ZHOU Quan;LYU Haoqiu;YU Liye;SUN Huizhong(Zhumadian Branch of Henan Provincial Tobacco Company,Zhumadian Henan 463000;College of Agriculture,Henan University of Science and Technology,Luoyang Henan 471000)

机构地区：[1]河南省烟草公司驻马店市公司,河南驻马店463000 [2]河南科技大学农学院,河南洛阳471000

出　　处：《现代农业科技》2023年第1期71-74,共4页Modern Agricultural Science and Technology

基　　金：河南省烟草公司驻马店市公司技术开发项目“烟草主要真菌病害多靶向微生态绿色防控技术研究与应用”(2022411700270011)。

摘　　要：从连作障碍较严重的烟草根际土壤中分离出49株细菌活体纯培养物,依次编号为HHHB1~HHHB49,并筛选出对烟草赤星病病原菌(Alternaria alternate)具有拮抗活性的菌株HHHB7和HHHB19,二者抑菌圈直径分别为5、23 mm。二者初步定性为具有生防活性。多相鉴定结果表明,活性最强的菌株HHHB19隶属于假单胞菌属(Pseudomonas),暂定名Pseudomonas sp. HHHB19。盆栽试验证实,HHHB19菌株悬液叶面喷雾混合处理对烟草赤星病不同发病阶段均表现出一定的防治效果,其中喷施14 d后防治效果达61.83%。菌株HHHB19可作为良好的生防菌备用资源。A total of 49 strains of live pure cultures were isolated from tobacco rhizosphere soil with serious continuous cropping obstacles, which were numbered from HHHB1 to HHHB49, respectively, and the strains of HHHB7 and HHHB19 with antagonistic activity against Alternaria alternate were screened out. The diameters of bacteriostatic circles of the two strains were 5 mm and 23 mm, respectively. The two strains were preliminarily characterized as having biocontrol activity. The results of polyphasic identification showed that the most active strain(HHHB19) belonged to the genus of Pseudomonas, and its temporary name was Pseudomonas sp. HHHB19. Pot experiment confirmed that the mixed treatment of HHHB19 strain suspension foliar spray showed certain control effects against tobacco brown spot disease at different stages, and the control effect reached 61.83% after 14 days. HHHB19 strain can be used as a good reserve resource for biocontrol bacteria.

关 键 词：烟草赤星病 生防菌 分离鉴定 应用效果 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]