The nuclear export receptor OsXPO1 is required for rice development and involved in abiotic stress responses  

作　　者：Qiufei Peng Jieyu Qiu Xintong Li Xuezhong Xu Xinxiang Peng Guohui Zhu 

机构地区：[1]Guangdong Laboratory for Lingnan Modern Agriculture,College of Life Sciences,South China Agricultural University,Guangzhou 510640,Guangdong,China [2]Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms,Guangzhou 510640,Guangdong,China

出　　处：《The Crop Journal》2023年第1期71-78,共8页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program(2020YFA0907600);the Laboratory of Lingnan Modern Agriculture Project(NZ2021004);the Natural Science Foundation of Guangdong Province(2020A1515010157);the Science and Technology Program of Guangzhou(202102080499)。

摘　　要：The transport of proteins to and from the nucleus is necessary for many cellular processes and is one of the ways plants respond to developmental signals and environmental stresses.Nucleocytoplasmic trafficking of proteins is mediated by the nuclear transport receptor(NTR).Although NTR has been extensively studied in humans and Arabidopsis,it has rarely been identified and functionally characterized in rice.In this study,we identified exportin 1 in rice(OsXPO1)as a nuclear export receptor.OsXPO1shares high protein identity with its functional homologs in Arabidopsis and other organisms.OsXPO1localized to both the nucleus and the cytoplasm,directly interacted with the small GTPases OsRAN1and OsRAN2 in the nucleus,and mediated their nuclear export.Loss-of-function osxpo1 mutations were lethal at the seedling stage.Suppression of OsXPO1 expression in RNA interference lines produced multifaceted developmental defects,including arrested growth,premature senescence,abnormal inflorescence,and brown and mouth-opened spikelets.Overexpression of OsXPO1 in rice reduced plant height and seed-setting rate,but increased plant tolerance in response to PEG-mimicked drought stress and salt stress.These results indicate that OsXPO1 is a nuclear export receptor and acts in regulating plant development and abiotic stress responses.

关 键 词：Exportin 1 Nucleocytoplasmic transport Plant development Abiotic stress 

分 类 号：S511[农业科学—作物学]