Increased nitrogen use efficiency via amino acid remobilization from source to sink organs in Brassica napus  

作　　者：Guihong Liang Yingpeng Hua Haifei Chen Jinsong Luo Hongkun Xiang Haixing Song Zhenhua Zhang 

机构地区：[1]College of Resources and Environmental Sciences,Hunan Agricultural University,Changsha 410128,Hunan,China [2]School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450000,Henan,China

出　　处：《The Crop Journal》2023年第1期119-131,共13页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(U21A20236,32072664);Natural Science Foundation of Hunan Province(2021JJ0004);China Agriculture Research System,and the Hunan Postgraduate Scientific Research Innovation Project(CX20190505)。

摘　　要：Nitrogen(N)is an essential plant growth nutrient whose coordinated distribution from source to sink organs is crucial for seed development and overall crop yield.We compared high and low N use efficiency(NUE)Brassica napus(rapeseed)genotypes.Metabonomics and transcriptomics revealed that leaf senescence induced by N deficiency promoted amino acid allocation from older to younger leaves in the high-NUE genotype at the vegetative growth stage.Efficient source to sink remobilization of amino acids elevated the numbers of branches and pods per plant under a N-deficiency treatment during the reproductive stage.A15N tracer experiment confirmed that more amino acids were partitioned into seeds from the silique wall during the pod stage in the high-NUE genotype,owing mainly to variation in genes involved in organic N transport and metabolism.We suggest that the greater amino acid source-to-sink allocation efficiency during various growth stages in the high-NUE genotype resulted in higher yield and NUE under N deficiency.These findings support the hypothesis that strong amino acid remobilization in rapeseed leads to high yield,NUE,and harvest index.

关 键 词：Nitrogen Amino acids Source-to-sink allocation Yield Genetic variation 

分 类 号：S565.4[农业科学—作物学]