See the color,see the seed:GmW1 as a visual reporter for transgene and genome editing in soybean  

作　　者：Li Chen Shan Yuan Yupeng Cai Weiwei Yao Qiang Su Yingying Chen Jialing Zhang Wensheng Hou 

机构地区：[1]National Center for Transgenic Research in Plants,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《The Crop Journal》2023年第1期311-315,共5页作物学报（英文版）

基　　金：supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agriculture Sciences(S2022ZD03)。

摘　　要：A fast and efficient recognition method of transgenic lines will greatly improve detection efficiency and reduce cost.In this study,we successfully identified the transgenic soybean plants by the color.We isolated a GmW1 gene encoding a flavonoid 3'5'-hydroxylase from a soybean cultivar ZH42(purple flower).We found that purple flowers occurred in the overexpression lines in the Jack and Williams 82 backgrounds(white flower).All plants with purple flowers were positive,and this trait seems stably inherited in the offspring.We have also obtained the editing plants,which were classified into three types according to the different flower colors appeared.We analyzed the phenotype and the homozygous types of the T_1mutants.We also found that a correspondence between flower color and stem color.This study provides a visible color reporter on soybean transformation.It can be quickly and early to identify the transgenic soybean plants by stem color of seedlings,which substantially reduces the amount of labor and cost.

关 键 词：SOYBEAN GmW1 COLOR Transgenic lines Genome editing 

分 类 号：S565.1[农业科学—作物学]