天南星的化学成分研究  

Chemical constituents of Arisaema erubescens(Wall.)Schott

作　　者：罗雨萌 孙延平[1] 黎冠兰 杨炳友[1] 匡海学[1] LUO Yu-meng;SUN Yan-ping;LI Guan-lan;YANG Bing-you;KUANG Hai-xue(Key Laboratory of Basic Research and Application of Northern Medicine,Heilongjiang University of Chinese Medicine,Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin 150040)

机构地区：[1]黑龙江中医药大学省部共建教育部北药基础与应用研究重点实验室/黑龙江省中药及天然药物药效物质基础研究重点实验室/黑龙江中医药大学药学院,哈尔滨150040

出　　处：《中南药学》2022年第12期2818-2822,共5页Central South Pharmacy

基　　金：国家中医药管理局中医药传承与创新“百千万”人才工程——岐黄工程首席科学家支持项目(编号:国中医药人教函﹝2021﹞7号);国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者支持项目(编号:国中医药人教函﹝2018﹞284号);2022年全国名老中医药专家传承工作室建设项目(编号:国中医药人教函〔2022〕75号);第七批全国老中医药专家学术经验继承工作项目(编号:国中医药人教函〔2022〕76号);黑龙江省“头雁”团队支持项目(编号:黑龙江省头雁行动领导小组文件﹝2019﹞5号)。

摘　　要：目的研究天南星的化学成分。方法采用硅胶、ODS、Sephadex LH-20等柱色谱法以及制备型HPLC对天南星70%乙醇提取液进行分离纯化,运用HR-ESI-MS和NMR等多种波谱学方法对化合物结构进行鉴定。结果从天南星中分离并鉴定了10个化合物,分别为cis-coniferin(1),coniferin(2),methylconiferin(3),icarisides F2(4),3-methylbutan-1-olβ-Dglucopyranoside(5),n-hexyl O-β-D-glucopyranoside(6),apigenin-7-O-neohesperidoside(7),kaempferol 3-O-rutinoside(8),pinoresinol-4-O-β-D-glucoside(9),pinoresinol(10)。结论化合物1~8为首次从天南星科植物中分离得到,其中化合物7、8对脂多糖诱导的BV-2细胞的炎症反应具有抑制作用。Objective To determine the chemical constituents from Arisaema erubescens(Wall.)Schott.Methods Silica gel,ODS,Sephadex LH-20 column chromatography and preparative HPLC were used to separate and purify the 70%ethanol extract of Arisaema ammrense(Wall.)Schott.HR-ESI-MS,NMR and other spectroscopic methods were used to identify the structure of the compounds.Results Ten compounds were obtained from Arisaema amurense(Wall.)Schott and identified as cis-coniferin(1),coniferin(2),methylconiferin(3),icarisides F2(4),3-methylbutan-1-olβ-D-glucopyranoside(5),n-hexyl O-β-D-glucopyranoside(6),apigenin-7-O-neohesperidoside(7),kaempferol 3-O-rutinoside(8),pinoresinol-4-O-β-D-glucoside(9),and pinoresinol(10).Conclusion Compounds 1~8 have been isolated from Araceae for the first time.Compounds 7 and 8 can inhibit lipopolysaccharide induced inflammation in BV-2 microglial cells.

关 键 词：天南星 化学成分 糖苷 黄酮 木脂素 

分 类 号：R284.1[医药卫生—中药学]