基于网络药理学的多叶棘豆总黄酮抗炎作用机制研究  

Study on the Anti-inflammatory Mechanism of Total Flavonoids in Oxytropis Myriophylla Based on Network Pharmacology

作　　者：杨立国 郝俊生 萨其拉吐 苏都那布其 王秀兰[1,2] YANG Li-guo;HAO Jun-sheng;Saqilatu;Sudunabuqi;WANG Xiu-lan(College of Mongolian Medicine and Pharmacy,Inner Mongolia Minzu University,Tongliao 028043,China;Mongolian Medicine Engineering Technology Institute of Inner Mongolia,Tongliao 028000,China;Tongliao Institute for Food and Drug Control,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028043 [2]内蒙古蒙医药工程技术研究院,内蒙古通辽028000 [3]通辽市食品药品检验所,内蒙古通辽028000

出　　处：《内蒙古民族大学学报（自然科学版）》2022年第6期506-512,517,共8页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：内蒙古自治区蒙药工程研究中心开放基金项目(MDK2017073)。

摘　　要：目的:通过网络药理学探讨多叶棘豆总黄酮抗炎作用机制。方法:通过Pub Chem及Swiss Target Prediction数据库对蒙药多叶棘豆中24个黄酮类成分的潜在靶点进行预测,利用Gene Cards数据库检索炎症相关靶点,将以上两个靶点结果取交集,利用String数据库构建PPI网络,在DAVID平台进行GO功能富集和KEGG通路富集分析,利用Cytoscape软件构建化学成分-靶点-疾病网络图。结果:多叶棘豆24个黄酮类成分共得到350个靶点,与炎症有关的靶点取交集后共得到18个共有靶点,GO分析共得到59个条目,KEGG分析得到9条信号通路,化学成分-靶点-疾病网络显示多叶棘豆总黄酮抗炎核心成分有5种,包括5,4’-dihydroxy-7,3’-dimethoxyflavone、5,7-dihydroxy-6,4’-dimethoxyflavone、apigenin、luteolin、kaempferol等;核心靶点有5个,包括ABCB1、PTGS2、ALOX5、SYK、NOS2等。结论:网络药理学直观地反映了多叶棘豆总黄酮可能通过多成分、多靶点和多通路来治疗炎症性疾病,为深入研究多叶棘豆总黄酮抗炎作用机制提供了科学依据。Objective:To explore the anti-inflammatory mechanism of total flavonoids of Oxytropis myriophylla through network pharmacology.Methods:The potential targets of 24 flavonoids from Oxytropis myriophylla were predicted by Pub Chem database and Swiss Target Prediction database.The inflammatory related targets were retrieved by Gene Cards database.The intersection of the above two targets was obtained,and the PPI network was constructed by String database.GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on DAVID platform,and chemical component-target-disease network was constructed using Cytoscape software.Results:A total of 350 effective targets were obtained from 24 flavonoids,18 common targets were obtained from the intersection of inflammatory related targets,59 items were obtained from GO enrichment analysis,9 signaling pathways were obtained from KEGG enrichment analysis,and 5 core anti-inflammatory components of total flavonoids were found from chemical component-target-disease network,including 5,4’-dihydroxy-7,3’-dimethoxyflavone,5,7-dihydroxy-6,4’-dimethoxyflavone,apigenin,luteolin and kaempferol,and 5 core anti-inflammatory targets were found,including ABCB1,PTGS2,ALOX5,SYK and NOS2.Conclusion:The network pharmacology directly reflects that total flavonoids of Oxytropis myriophylla may treat inflammatory diseases by multiple components,multiple targets and mutiple pathways,and provides reference for further study of antiinflammatory mechanism of total flavonoids of Oxytropis myriophylla.

关 键 词：网络药理学 多叶棘豆总黄酮 炎症 作用机制 

分 类 号：R692[医药卫生—泌尿科学]