DNA加合物组的预处理及检测方法研究进展  

Recent progress in the preprocessing and detection of DNA adductomics

作　　者：李昕冉 孙铜 郭娜 张峻颖[5] 吴春勇[1,2] LI Xinran;SUN Tong;GUO Na;ZHANG Junying;WU Chunyong(Key Laboratory of Drug Quality Control and Pharmacovigilance,Ministry of Education,China Pharmaceutical University,Nanjing 210009,China;Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 211198,China;Shandong Institute for Food and Drug Control,Jinan 250101,China;Jinan Food and Drug Inspection and Testing Center,Jinan 250102,China;Department of Pharmaceutics of Traditional Chinese Medicine,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]中国药科大学药物质量与安全预警教育部重点实验室,江苏南京210009 [2]中国药科大学药物分析教研室,江苏南京211198 [3]山东省食品药品检验研究院,山东济南250101 [4]济南市食品药品检验检测中心,山东济南250102 [5]中国药科大学中药制剂教研室,江苏南京211198

出　　处：《药学研究》2022年第12期825-831,共7页Journal of Pharmaceutical Research

基　　金：国家自然科学基金(No.82074128、81673681、81473357);江苏省市场监督管理局科技计划项目(No.KJ207558);国家药品监督管理局化学药品杂质谱研究重点实验室开放课题(No.NMPA-KLIPCD-2020-01)。

摘　　要：DNA加合物为亲电性物质与DNA共价结合形成的一类DNA损伤,若不能及时修复可能会引发癌症。具有高灵敏度、高选择性的液质联用技术已成为DNA加合物检测的主流方法。DNA加合物组学能同时测定复杂生物基质中多种已知的DNA加合物,扫描和鉴定未知DNA加合物,因此可以更全面的阐释遗传毒性物质的毒性。虽然近年来在DNA水解、加合物纯化富集、液相分离、质谱扫描策略和数据处理等方面不断优化,DNA加合物组学检测仍面临着巨大的挑战。DNA adduct is a kind of DNA damage formed by the covalent combination of electrophilic substances and DNA.The unrepaired DNA damage might increase the risk of cancer.Liquid chromatography-mass spectrometry with high sensitivity and selectivity has been the most used method for the detection of DNA adducts.Multiple known or unknown DNA adducts could be detected and identified from the complex biological matrix with DNA adductomic technology, which could deepen the understanding of the toxicity of genotoxic substances.Although the sample pretreatment, detection technology and data processing have been optimized, DNA adductomic analysis still faces great challenges.

关 键 词：DNA加合物 遗传毒性 DNA水解 液质联用 

分 类 号：R917[医药卫生—药物分析学]