游离氨对EBPR系统聚磷菌活性抑制动力学  

Inhibitory Kinetics of Free Ammonia on the Activity of Polyphosphate Accumulating Organisms in EBPR Process

作　　者：牛永健 李维维 张昕 孙洪伟 NIU Yongjian;LI Weiwei;ZHANG Xin;SUN Hongwei(Gansu Research Institute of Light Industry Co.Ltd.,Lanzhou 730000,China;School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;School of Environmental and Material Engineering,Yantai University,Yantai 264003,China)

机构地区：[1]甘肃省轻工研究院有限责任公司,甘肃兰州730000 [2]兰州交通大学环境与市政工程学院,甘肃兰州730070 [3]烟台大学环境与材料工程学院,山东烟台264003

出　　处：《环境科学与技术》2022年第10期137-143,共7页Environmental Science & Technology

基　　金：甘肃省科技计划项目重点研发计划-社会发展类(21YF5FA045);山东省自然科学基金项目(ZR2020ME226);烟台大学博士启动基金项目(HJ19B92:1115/2219014)。

摘　　要：在强化生物除磷系统中,游离氨(FA)是影响聚磷菌(PAOs)生物除磷效果及微生物种群特性的重要因素之一。该试验以富含PAOs的活性污泥为研究对象,基于批次试验,考察不同FA浓度对EBPR系统除磷性能的影响,同时拟合了3种非基质抑制动力学模型(Andrew模型、Hellinga模型、Vadivelu模型)。试验结果表明:EBPR系统中Ca.Accumulibacter和Tetrasphaera菌属的相对丰度分别为2.65%和1.71%,是PAOs群落中主导的菌属。此外,当0 mg/L≤FA≤0.2 mg/L时,FA对PAOs的释磷过程和吸磷过程产生促进作用,当0.2 mg/L≤FA≤50 mg/L时,FA显著地抑制了PAOs的释磷过程和吸磷过程,且吸磷过程受抑制程度更强。最后,统计学参数分析发现,相对于Andrew和Vadivelu模型,Hellinga模型最适合描述FA对PAOs活性的抑制影响,获得了动力学常数。厌氧释磷过程:最大比释磷速率(r_(max),AN)为(17.6±0.5)mg/(g VSS·h),抑制常数(KI,AN)为(25.6±3.84)mg/L。好氧吸磷过程:最大比吸磷速率(r_(max),OX)为(6.3±0.19)mg/(g VSS·h),抑制常数(KI,OX)为(7.0±0.85)mg/L。该研究为优化生物除磷工艺的设计和运行提供了理论支持。In enhanced biological phosphorus removal(EBPR)process,free ammonia(FA)was an important factor that affecting phosphorus removal and microbial community characteristics.Enriched polyphosphate accumulating organisms(PAOs)was used to investigate the effect of FA on the performance of EBPR based on batch tests,and the relationships between FA and phosphorus removal were fitted by using Andrew,Hellinga,Vadivelu models,respectively.The results showed that the relative abundances of Ca.Accumulibacter and Tetrasphaera were 2.65%and 1.71%,respectively,of the total bacterial abundance,and were predominant PAOs in EBPR process.Both phosphorus release and phosphorus uptake processes were promoted within FA range of 0~0.2 mg/L,however,were inhibited within FA range of 0.2~50 mg/L,and phosphorus release process was sensitive to FA.Furthermore,statistical parameters clearly indicated that Hellinga’s model was the most optimal for description of the FA inhibition on the PAOs activity,and the kinetics parameters were obtained.The maximum specific rates and half-saturation inhibition coefficients were(17.6±0.5)mg/(g VSS·h)and(25.6±3.84)mg/L,respectively,for phosphorus release process,were(6.3±0.19)mg/(g VSS·h)and(7.0±0.85)mg/L,respectively,for phosphorus uptake process.Overall,the findings of this study could provide available theory guidance for design and operation of EBPR system in wastewater treatment plants.

关 键 词：游离氨 强化生物除磷 聚磷菌 动力学模型 

分 类 号：X703[环境科学与工程—环境工程]