多脂鳞伞多糖对H22荷瘤小鼠抗肿瘤作用  

Antitumor Effect of Pholiota adiposa Polysaccharide on H22 Tumor-Bearing Mice

作　　者：王晓岩 李刚[1] 孔凡丽 WANG Xiaoyan;LI Gang;KONG Fanli(Institute of Agricultural Quality Standard and Testing Technology,Jilin Academy of Agricultural Sciences,Changchun 130033,Jilin,China;College of Traditional Chinese Medicine,Jilin Agricultural Science and Technology University,Jilin 132109,Jilin,China)

机构地区：[1]吉林省农业科学院农业质量标准与检测技术研究所,吉林长春130033 [2]吉林农业科技学院中药学院,吉林吉林132109

出　　处：《食用菌学报》2023年第1期45-52,共8页Acta Edulis Fungi

基　　金：吉林省科技厅项目(20220508074RC);吉林省教育厅项目(JJKH20220397KJ)。

摘　　要：采用水提醇沉法获得多脂鳞伞(Pholiota adiposa)多糖(PAP),研究其对H22荷瘤小鼠的抗肿瘤作用。将小鼠随机分为模型组、阳性组(环磷酰胺CTX,25 mg·kg^(-1))及PAP低(100 mg·kg^(-1))、中(200 mg·kg^(-1))、高(300 mg·kg^(-1))剂量组,连续给药15 d,以灌胃生理盐水为空白组,计算抑瘤率和脏器指数;采用ELISA法测定小鼠血清中IFN-γ、IL-2、IL-6、TNF-α和VEGF含量,采用HE染色法观察肿瘤组织细胞排序、细胞完整度、细胞核数量,采用TUNEL法检测肿瘤组织细胞凋亡程度,采用免疫组化法检测VEGF、Bcl-2和BAX蛋白表达强度。结果表明:PAP具有抑制肿瘤生长的作用,高剂量组抑瘤率高达78.05%,并且对小鼠胸腺、脾脏等器官指数具有明显恢复作用;模型组小鼠肿瘤组织细胞排列整齐紧密,生长状态良好,凋亡率较低,PAP处理组小鼠的肿瘤组织细胞出现不同程度的细胞变形、破裂及模糊不清等现象,并且出现大面积凋亡;PAP可提高小鼠血清中IFN-γ、IL-2、IL-6含量,降低VEGF含量,通过影响VEGF、Bcl-2和BAX的表达水平而表现出较好的抗肿瘤作用。Using water extraction and alcohol precipitation, polysaccharide of Pholiota adiposa(PAP) was obtained and then studied for its antitumor effect on H22 tumor-bearing mice. ICR mice were randomly divided into five groups as follows: model group, positive group(cyclophosphamide, 25 mg·kg^(-1)·d^(-1)), low-dose PAP group(LD, 100 mg·kg^(-1)·d^(-1)), medium-dose PAP group(MD, 200 mg·kg^(-1)·d^(-1)) and high-dose PAP group(HD, 300 mg·kg^(-1)·d^(-1)). Using gavage with saline as the blank control, mice in different groups were measured for tumor suppression rate and organ indices after 15 consecutive days of treatment. For each group, the contents of IFN-γ, IL-2, IL-6, TNF-α and VEGF in mouse serum were determined by ELISA;tumor cell arrangement, cell integrity, number of nuclei were observed by hematoxylin-eosin(HE) staining;the degree of apoptosis in tumor tissues was determined by TUNEL assay;and the expression of VEGF, Bcl-2 and BAX was determined by immunohistochemistry. The results showed that PAP inhibited tumor growth, and the tumor inhibition rate of HD group reached 78.05%, with organ indices such as thymus index and spleen index significantly improved.In the model group, the tumor cells were arranged neatly and tightly, grew well, and the apoptosis rate was low. In contrast, the tumor cells of the PAP treatment groups were deformed, broken and blurred at different degrees, with large areas of apoptosis. PAP increased the contents of IFN-γ, IL-2 and IL-6 in mouse serum, decreased VEGF level, and showed anti-tumor activity through regulating the expression of VEGF, Bcl-2and BAX.

关 键 词：多脂鳞伞 抗肿瘤 细胞凋亡 生化指标 

分 类 号：R285.5[医药卫生—中药学]