粗毛纤孔菌与桑叶发酵产物——粗桑茶对H22荷瘤小鼠抗肿瘤作用  

Antitumor Effect of a Fermentation Product of Inonotus hispidus and Mulberry Leaf on H22 Tumor-Bearing Mice

作　　者：罗诗蓉 包海鹰[1] LUO Shirong;BAO Haiying(College of Chinese Materia Medica&Key Research Laboratory for the Development and Utilization of Fungi Traditional Chinese Medicine Resources,Jilin Agricultural University,Changchun 130118,Jilin,China)

机构地区：[1]吉林农业大学中药材学院,菌类中药资源开发与利用重点研究室,吉林长春130118

出　　处：《食用菌学报》2023年第1期53-63,共11页Acta Edulis Fungi

基　　金：吉农德百桑黄研究与产业开发中心(20210080)。

摘　　要：以桑叶为培养基质,利用粗毛纤孔菌(Inonotus hispidus)菌株进行发酵,制备粗桑茶,用粗桑茶(低、中、高剂量分别为195、390、780 mg·kg^(-1))灌胃H22荷瘤小鼠,测定小鼠体质量、肿瘤体积、肿瘤抑制率和胸腺、肝脏、肾脏、脾脏指数,检测血清中IL-2、IL-6、TNF-α、IFN-γ含量,用苏木素-伊红染色观察小鼠肝脏、肾脏、脾脏、肿瘤组织,用免疫组化染色观察小鼠肿瘤组织,并采用Western blot方法检测肿瘤相关蛋白表达情况。结果表明:与模型组相比,粗桑茶各剂量组肿瘤体积均极显著减小;粗桑茶高剂量组小鼠肿瘤质量极显著降低;粗桑茶高剂量组胸腺指数极显著升高;粗桑茶低剂量组小鼠血清中的IL-2、IL-6,中剂量组的TNF-α,高剂量组的IL-2含量显著降低;粗桑茶低剂量组的TNF-α、IFN-γ,中剂量组的IL-2、IFN-γ,高剂量组的IL-6、TNF-α、IFN-γ含量显著增加;粗桑茶各剂量组小鼠肿瘤组织的Bax、Cytc、Parp、TNF-α、caspase-3、cleaved caspase-3、caspase-9、 cleaved caspase-9蛋白表达量显著增加,Bcl-2蛋白表达量显著降低。研究结果表明粗桑茶具有抗肿瘤活性,说明采用粗毛纤孔菌菌株发酵桑叶制备产品的思路可行,为进一步工业化生产提供参考。Using mulberry leaves(Mori folium) as the culture substrate, Inonotus hispidus was fermented to yield Cusang tea, which was then studied for its antitumor activity. Low(195 mg·kg^(-1)), medium(390 mg·kg^(-1)) and high(780 mg·kg^(-1)) doses of Cusang tea were administered to H22 tumor-bearing mice by gavage for 15 d, and then mouse body mass, tumor volume, tumor inhibition rate, thymus index, spleen index, kidney index, serum levels of IL-2, IL-6, TNF-α, and IFN-γ were tissues measured;liver, kidney, and spleen tissues were observed by HE staining;pathological sections of tumor tissues were observed by both HE staining and immunohistochemistry;and the expression of tumor related proteins was detected by Western blot. The results showed that compared with the model group, mice administered Cusang tea at all doses had extremely significantly reduced tumor size, and mice administered high dose Cusang tea showed an extremely significant decrease in tumor mass. The thymus index of mice administered high dose Cusang tea increased significantly(P<0.01) when compared with that of the model group. There was a significant decrease of serum IL-2 and IL-6, as well as a significant increase of TNF-α and IFN-γ in mice administered low dose Cusang tea.For mice administered medium dose Cusang tea, there was a significant decrease of TNF-α and a significant increase of IL-2 and IFN-γ when compared with the model group. Mice administered high dose Cusang tea showed significantly higher IL-6, TNF-α, IFN-γ, and significantly lower IL-2. The expression levels of Bax, Cytc, Parp, TNF-α, caspase-3, cleaved caspase-3, caspase-9 and cleaved caspase-9 proteins were significantly increased in mice administered Cusang tea, whereas the expression of Bcl-2 was significantly decreased in mice administered Cusang tea. These findings suggested that fermentation of I. hispidus with mulberry leaves has the potential to develop antitumor products.

关 键 词：粗毛纤孔菌 桑叶 H22荷瘤小鼠 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]