F-53B经饮水慢性暴露对SD大鼠甲状腺功能影响的研究  

Influence of chronic perfluoroalkyl ether sulfonate exposure via drinking water on thyroid function of Sprague-Dawley rats

作　　者：单珊[1] 李晓涵 陈晓玉 王爱清[2] 王亚荣[2] 宁萍[2] 王雪莹 田海林[1] SHAN Shan;LI Xiao-han;CHEN Xiao-yu;WANG Ai-qing;WANG Ya-rong;NING Ping;WANG Xue-ying;TIAN Hai-lin(School of Public Health,Suzhou Medical College,Soochow University,Suzhou,Jiangsu Province 215123,China;Experimental Center,Suzhou Medical College,Soochow University)

机构地区：[1]苏州大学苏州医学院公共卫生学院,江苏苏州215123 [2]苏州大学苏州医学院实验中心

出　　处：《环境卫生学杂志》2022年第12期856-861,共6页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家自然科学基金(81673126)。

摘　　要：目的探讨全氟烷基醚磺酸盐(F-53B)经饮水慢性暴露对SD大鼠甲状腺功能的影响。方法将40只SPF级雄性SD大鼠随机分为溶剂对照组(二甲亚砜)和暴露组(1、10、100、1000μg/L F-53B),每组8只,各组二甲亚砜终浓度均为0.004%(体积分数),从2020年6月起自由饮水暴露F-53B 6个月。检测大鼠血清中总胆固醇(TCHO)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量,分析血清四碘甲状腺原氨酸(T4)、三碘甲状腺原氨酸(T3)、促甲状腺激素(TSH)、促甲状腺素释放激素(TRH)和甲状腺过氧化物酶抗体(TPO-Ab)激素水平;进行甲状腺组织病理学观察。结果F-53B暴露6个月后,各暴露组大鼠体重、甲状腺重量和脏器系数差异无统计学意义(F值为0.61~2.20,P>0.05)。与对照组相比,血清中TCHO、HDL-C、LDL-C在各暴露组中差异并无统计学意义(F值为0.26~0.84,P>0.05);甲状腺相关激素在各暴露组中差异具有统计学意义(F值为30.4~131.3,P<0.05),其中T3、T4水平随着暴露剂量的升高而降低,TSH、TRH和TPO-Ab则呈剂量依赖性升高,10、100和1000μg/L暴露组差异有统计学意义(校正后P<0.05)。病理结果显示,最高剂量组大鼠甲状腺组织呈现轻度甲状腺滤泡增生。结论F-53B慢性暴露可导致大鼠甲状腺组织的结构性改变和功能性异常,可能与甲状腺激素合成过程中碘甲状腺原氨酸的耦合受到干扰有关。Objective To investigate the influence of chronic exposure to perfluoroalkyl ether sulfonate(F-53B)via drinking water on the thyroid function of Sprague-Dawley(SD)rats.Methods A total of 40 specific pathogen-free male SD rats were randomly divided into solvent control group(dimethyl sulfoxide,DMSO)and exposure groups(1,10,100,and 1000μg/L F-53B),with 8 rats in each group.The final concentration of DMSO was 0.004%(volume fraction)for each group,and the rats were exposed to F-53B through drinking water for 6 months since June 2020.The serum levels of total cholesterol(TCHO),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)were measured,as well as the serum levels of tetraiodothyroxine(T4),triiodothyronine(T3),thyroid stimulating hormone(TSH),thyrotropin-releasing hormone(TRH),and thyroid peroxidase antibody(TPO-Ab).Histopathological changes of the thyroid were also observed.Results After 6 months of F-53B exposure,there were no significant differences among the exposure groups in body weight,thyroid weight,and organ coefficient of rats in each exposure group(F=0.61-2.20,P>0.05).Compared with the control group,there were no significant differences in the serum levels of TCHO,HDL-C,and LDL-C among the exposure groups(F=0.26-0.84,P>0.05).There were significant differences in thyroid-related hormones between the exposure groups(F=30.4-131.3,P<0.05),among which T3 and T4 decreased with the increase in the exposure dose of F-53B,while the levels of TSH,TRH and TPO-Ab increased in a dose-dependent manner,with significant differences among the 10,100,and 1000μg/L exposure groups(adjusted P<0.05).Pathological result showed mild thyroid follicular hyperplasia in thyroid tissue in the highest dose group.Conclusion Chronic F-53B exposure can induce structural alterations and functional abnormalities in the thyroid tissue of rats,which may be associated with the disturbance of iodothyronine coupling during thyroid hormone synthesis.

关 键 词：全氟烷基醚磺酸盐(F-53B) SD大鼠 甲状腺 慢性暴露 

分 类 号：R12[医药卫生—环境卫生学]