施加硒肥条件下的印度梨形孢接种对香榧幼苗根系发育、气体交换及其叶片硒累积的影响  

Effects of Piriformospora indica inoculation on root development,growth and selenium accumulation in leaves of Torreya grandis seedlings supplied with selenium fertilizer

作　　者：李爱华[1] 许秀环[1] 李金柱[1] 蒲尧 吴楚[2] LI Aihua;XU Xiuhuan;LI Jinzhu;PU Yao;WU Chu(Hubei Institute of Forestry,Wuhan 430075;College of Horticulture&Gardening,Yangtze University,Jingzhou 434025)

机构地区：[1]湖北省林业科学研究院,武汉430075 [2]长江大学园艺园林学院,荆州434025

出　　处：《安徽农业大学学报》2022年第5期735-740,共6页Journal of Anhui Agricultural University

基　　金：湖北省林业科技支撑重点项目(2016-LYKJ01)资助。

摘　　要：近些年来香榧果实在干果市场非常受到人们的喜爱,如何提高其果实产量,促进其果实品质尤其微量元素的强化是当前香榧栽培上需要解决的问题。微量元素硒是硒蛋白必需的元素,它与多种人体疾病有关。采用盆栽的方式,施加矿物硒粉,并对香榧幼苗接种根内生真菌印度梨形孢。结果表明,印度梨形孢可以侵染香榧幼苗的根系(侵染率为22.34%),并使其总根长、总根表面积和总根体积以及二级根上一级根的数量,分别增加12.69%、16.26%、20.81%和10.93%,但对其特定根长和根组织密度没有显著影响。香榧幼苗接种印度梨形孢可显著提高净光合速率、气孔导度、胞间二氧化碳浓度以及蒸腾速率,分别增加92.92%、6.16倍、47.67%和2.36倍,同时显著提高光能利用效率(88.62%)而降低水分利用效率(84.28%)。接种显著地促进了香榧幼苗总生物量的累积(36.94%)以及叶片总硒含量(1.68倍)。基于印度梨形孢可促进植物营养吸收的特性,该根内生真菌可以用香榧硒的生物强化,以提高香榧果实的品质。In recent years,fruits of Torreya grandis enjoy great popularity,now,how to increase fruit yield and improve fruit quality,especially the concentrations of micronutrients in fruits are essential for T.grandis cultivation.Selenium is the essential element for the biosynthesis of selenoproteins,and lack of it is related to some human diseases.In the present study,T.grandis seedlings were planted in plastic pots,powder of selenium rock was applied in the cultivation substrate,and root endophytic fungus Piriformospora indica was inoculated with T.grandis seedlings.The results showed the fungus could infect the roots of T.grandis seedlings,and the inoculation increased the total root length,surface areas,root volumes and the numbers of the first-order roots on the second-order roots by 12.69%,16.26%,20.81%and 10.93%,respectively.However,the inoculation did not affect the specific root length and root tissue densities of T.grandis seedling roots.The inoculation significantly increased the net photosynthesis rates,stomatal conductance,intercellular CO2concentrations and transpiration rates by 92.92%,6.16 times,47.67%and 2.36 times,respectively.And the inoculation significantly increased the light use efficiency(88.62%)and reduced the water use efficiency(84.28%).Compared with non-inoculated seedlings,inoculated seedlings showed significantly higher total biomass accumulation and total selenium concentrations in the leaves by 36.94%and 1.68 times,correspondingly.The result suggested that the root endophytic fungus P.indica might be used in cultivation of T.grandis for selenium biofortification in the fruits.

关 键 词：硒 香榧 印度梨形孢 生物强化 

分 类 号：S791.53[农业科学—林木遗传育种]