半野生番茄GZ-01砧木增强嫁接植株耐旱性  

Semi-wild Tomato GZ-01 Rootstock Enhances Drought Tolerance of Grafted Plant

作　　者：韦建明 黄鑫 米娜 张大龙 李云洲 WEI Jianming;HUANG Xin;MI Na;ZHANG Dalong;LI Yunzhou(College of Agriculture,Guizhou University,Guiyang 550025,Guizhou,China;College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018,Shandong,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《中国蔬菜》2023年第1期42-51,共10页China Vegetables

基　　金：贵州省科技计划项目〔黔科合基础-ZK(2022)一般071〕;国家自然科学基金项目(32060679);贵州大学培育项目〔贵大培育(2019)52号〕。

摘　　要：以贵州半野生番茄GZ-01为砧木,以红果番茄R、粉果番茄P为接穗,使用20%PEG-6000溶液模拟干旱胁迫处理未嫁接(R、P、GZ-01)、嫁接(RGZ-01、PGZ-01)和自嫁接(RR、PP)番茄植株,对嫁接植株生长生理指标、气孔开闭状态、防御酶活性、活性氧积累以及MAPKs和ABA合成相关基因的表达进行分析。结果表明,干旱处理下,GZ-01砧木能够增强嫁接植株防御酶、抗氧化酶活性,降低叶片气孔开放状态比例和H_(2)O_(2)、O_(2)^(-).积累量,诱导嫁接植株MAPKs、ABA相关基因的表达,以增强嫁接植株的耐旱性,且耐旱性强弱与接穗植株相关。Taking Guizhou semi-wild tomato GZ-01 as rootstock,red fruit tomato R and pink fruit tomato P as scion,this experiment used 20%PEG-6000 solution as simulate drought stress to treat un-grafted(R,P,GZ-01),grafted(RGZ-01,PGZ-01)and self-grafted(RR,PP)tomato plants;and carried out analysis on the growth physiological indexes,stomatal opening and closing,defense enzyme activity,active oxygen accumulation and expression of MAPKs and ABA synthesis related genes.The results showed that under drought treatment,GZ-01 rootstock could enhance the activities of defense enzymes and antioxidant enzymes in grafted plants;reduce open state proportion of stomata and H_(2)O_(2) and O_(2)^(-).accumulation in leaf blades;and induce the expression of MAPKs and ABA-related genes in grafted plants,so as to enhance the drought tolerance of grafted plants.Drought tolerance was related to scion plants,no matter it was strong or weak.

关 键 词：番茄 嫁接 干旱 丝裂原活化蛋白激酶 脱落酸 活性氧 

分 类 号：S641.2[农业科学—蔬菜学]