hucMSC-exo介导的mir-124对大鼠肝再生的影响及机制  

Effect and Mechanism of HucMSC-exo-mediated Mir-124 on Liver Regeneration in Rats

作　　者：刘旺 陈梅 肖经华 许芝彬 Liu Wang;Chen Mei;Xiao Jinghua;Xu Zhibin(Department of Internal Medicine,Panyu Fifth People's Hospital;Department of Ultrasound,Panyu Fifth People's Hospital,Guangzhou 510000 China;Faculty of Science,University Tunku Abdul Rahman,Kampar 50744 Malaysia)

机构地区：[1]广州市番禺区第五人民医院内科 [2]广州市番禺区第五人民医院超声科,广东广州510000 [3]马来西亚拉曼大学理学院,马来西亚坎帕尔50744

出　　处：《锦州医科大学学报》2022年第6期32-39,共8页Journal of Jinzhou Medical University

基　　金：2021年番禺区科技计划医疗卫生项目立项,项目编号:2021-Z04-012。

摘　　要：目的分子生物学hucMSC-exo传递mir-124增进大鼠肝再生的调节作用及机制。方法采用大鼠构建动物模型,构建过表达载体及转染,采用BrdU检测及定时定量PCR。结果术后注射组和未注射组的表达关系,mir-193a、mir-204、mir-124以及不同组间mir-93、mir-10a-5p表达存在着差异,mir-124组间有明显的表达差异。结论hucMSCs衍生的外泌体mir-124通过负性调节大鼠部分肝切除模型中的Foxg1,促进肝再生并抑制肝损伤,这些发现可用于开发肝再生的新疗法。Objective To study the regulatory role and mechanism of hucMSC-exo transduction of mir-124 in promoting rat liver regeneration.Methods Rats were used to construct the animal model,and the overexpression vector was constructed and transfected.BrdU detection and timed quantitative PCR were used.Results Expression relationship between injected and non-injected groups after surgery:There were differences in the expression of mir-193a,mir-204,mir-124,mir-93,mir-10a-5p among different groups,and there was a significant difference in the expression of mir-124 among different groups.Conclusion HucMSCs-derived exosomal mir-124 promotes liver regeneration and inhibits liver injury by negatively regulating Foxg1 in a rat partial hepatectomy model.These findings can be used to develop new therapies for liver regeneration.

关 键 词：大鼠 外泌体 肝再生 

分 类 号：Q418[生物学—生理学]