PGRN通过TGF-β1调控皮肤损伤后纤维形成  

PGRN regulated the fibrogenesis during skin wound healing via TGF-β1

作　　者：赵春明 张美香 刘佳林 李周儒 马静媛 李姗姗 Zhao Chunming;Zhang Meixiang;Liu Jialin;Li Zhouru;Ma Jingyuan;Li Shanshan(Department of human anatomy,Xuzhou Medical University,Xuzhou,Jiangsu,221006,China;Forensic identification of justice,Taixing people’s hospital,Taixing,Jiangsu,225400,China;Department of Forensic Medicine,Xuzhou Medical University,Xuzhou,Jiangsu,221006,China)

机构地区：[1]徐州医科大学人体解剖学教研室,江苏徐州221006 [2]泰兴市人民医院司法鉴定所,江苏泰兴225400 [3]徐州医科大学法医学教研室,江苏徐州221006

出　　处：《齐齐哈尔医学院学报》2022年第22期2105-2112,共8页Journal of Qiqihar Medical University

基　　金：徐州医科大学优秀人才科研启动基金(D2016004)。

摘　　要：瘢痕是皮肤损伤愈合的必然产物,前期研究表明,沉默内源性颗粒蛋白前体(PGRN)促进胶原沉积和纤维形成。本研究继续应用小鼠皮肤孔状切除模型,在创口及其周围皮下注射Si-m-PGRN和/或LV3-TGF-β1敲减PGRN和/或TGF-β1,研究TGF-β1在PGRN调控皮肤损伤愈合纤维形成中的作用。结果显示,LV3-TGF-β1和Si-m-PGRN可以较好的敲减TGF-β1和PGRN,敲减TGF-β1逆转了PGRN对纤维形成的调控,同时逆转PGRN对下游smad3信号通路的调控。提示PGRN可通过TGF-β1调控其下游信号通路影响皮肤损伤后纤维形成。A scar is an inevitable product of skin wound healing.Previous studies have shown that silencing endogenous progranulin(PGRN)promotes collagen deposition and fibrogenesis.To study the role of TGF-β1 in PGRN regulating skin wound healing,the Si-m-PGRN and/or LV3-TGF-β1 were injected subcutaneously around the wound to knock down PGRN and/or TGF-β1,using the mouse model of skin perforation continuously.The results showed that LV3-TGF-β1 and Si-m-PGRN may knock down TGF-β1 and PGRN effectively.The knock down of TGF-β1 interfered with the effect of PGRN on fibrogenesis and the downstream smad3 signal pathway.It is suggested that PGRN regulated fibrogenesis during wound healing through the TGF-β1 signal pathway.

关 键 词：皮肤 纤维形成 颗粒蛋白前体 转化生长因子 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]