健脾益气摄血方对原发免疫性血小板减少症模型小鼠凝血功能的影响  

Effect of Jianpi Yiqi Shexue Recipe on Coagulation Function in Mice with Primary Immune Thrombocytopenia

作　　者：严香 赵倩[1] 陈信义[1] 张雅月[1] YAN Xiang;ZHAO Qian;CHEN Xinyi;ZHANG Yayue(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《世界中医药》2022年第24期3481-3484,3490,共5页World Chinese Medicine

基　　金：国家重点基础研究发展计划项目(2013CB531705);国家自然科学基金青年科学基金项目(81703903);北京中医药大学东直门医院青苗人才培养计划项目(DZMYS-201807)。

摘　　要：目的:探讨健脾益气摄血方治疗原发免疫性血小板减少症(ITP)的可能作用机制。方法:将100只BALB/c小鼠随机分为正常组、模型组、泼尼松组、非从脾论治组、健脾益气摄血组,每组20只。在造模成功后的第8天,正常组、模型组小鼠给予生理盐水0.1 mL/10 g体积灌胃,泼尼松组给予浓度为2 mg/mL泼尼松溶液0.1 mL/10 g灌胃,中药组均按照0.1 mL/10 g体积灌胃给药,所有组给药1次/d,持续8 d。通过全自动血液凝固分析仪检测凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB),评估出血程度及指标变化。结果:与正常组比较,给药前各组出血程度显著升高,差异有统计学意义(P<0.01);药物干预第6、8天,与模型组比较,泼尼松组、健脾益气摄血组出血程度明显改善,差异有统计学意义(P<0.01)。与正常组比较,模型组小鼠PT、APTT显著升高,FIB水平下降,差异有统计学意义(P<0.01);与模型组比较,泼尼松组与健脾益气摄血组PT、APTT明显下降,FIB上升,差异有统计学意义(P<0.05或P<0.01);与非从脾论治组比较,健脾益气摄血组PT、APTT表达明显下降,FIB水平上升,差异有统计学意义(P<0.05或P<0.01)。结论:健脾益气摄血方能明显改善ITP小鼠出血程度及凝血功能。Objective:To explore the possible mechanism of Jianpi Yiqi Shexue Recipe(JYSR)in the treatment of primary immune thrombocytopenia(ITP).Methods:A total of 100 BALB/c mice were randomly divided into normal group,model group,prednisone group,non spleen treatment group,and JYSR group,with 20 in each group.On the 8th day after successful modeling,mice in the normal group and the model group were given 0.1 mL/10 g of normal saline by gavage,and those in the prednisone group were given 0.1 mL/10 g of prednisone solution with a concentration of 2 mg/mL by gavage.Mice in the non spleen treatment group and the JYSR group were treated with 0.1 mL/10 g corresponding drugs by gavage.The administration was performed once a day for 8 days.Prothrombin time(PT),activated partial thromboplastin time(APTT)and fibrinogen(FIB)were measured by full-automatic blood coagulation analyzer to evaluate the bleeding degree and index changes.Results:Before administration,the bleeding degree of each group was higher than that of the normal group(P<0.01).On the 6th and 8th days of drug intervention,the bleeding degree of the prednisone group and the JYSR group was improved compared with the condition in the model group(P<0.01).Compared with the conditions in the normal group,the PT and APTT in the model group were increased,while the FIB level was decreased(P<0.01).The PT and APTT in the prednisone group and the JYSR group were lower while the FIB level was higher than those in the model group(P<0.05 or P<0.01),and compared with the non spleen treatment group,the JYSR group had reduced PT and APTT while elevated FIB level(P<0.05 or P<0.01).Conclusion:JYSR can significantly improve the bleeding degree and coagulation function of ITP mice.

关 键 词：原发免疫性血小板减少症 从脾论治 止血机制 凝血功能 健脾益气摄血方 脾主统血 出血性疾病 

分 类 号：R285.5[医药卫生—中药学]