熔解曲线分析法对济南地区汉族人群HLA-A、B和HPA1-18,21基因多态性分布的研究  

Study on distribution of HLA-A,B and HPA1-18,21 gene polymorphisms in Han population in Jinan by melting curve analysis

作　　者：郝萧 李杰 刁雪芹 夏小叶 姚美雪 HAO Xiao;LI Jie;DIAO Xueqin;XIA Xiaoye;YAO Meixue(Jinan Blood Station,Jinan,250000,China;Department of Epidemiology and Biostatistics,School of Public Health,Xuzhou Medical University)

机构地区：[1]济南市血液供保中心,济南250000 [2]徐州医科大学公共卫生学院流行病学与生物统计学系

出　　处：《临床血液学杂志》2022年第12期888-891,896,共5页Journal of Clinical Hematology

摘　　要：目的:探讨济南地区汉族人群血小板抗原(HPA)1-18,21和人类白细胞抗原(HLA-A、B)的基因多态性分布,为建立本地区机采血小板献血者基因分型资料库奠定基础。方法:采用荧光PCR熔解曲线分析法对本站随机选取的347例无亲缘关系固定献血者进行HLA-A、B和HPA1-18,21基因分型。结果:HLA-A、B位点分别检测出18个和34个等位基因,基因频率较高的分布为A^(*)02(0.2781),A^(*)11(0.1686),A^(*)24(0.1470)与B^(*)13(0.1427)、B^(*)60(0.0692)、B^(*)61(0.0648)、B^(*)62(0.0634)。最常见的HPA基因组合型为:HPA-1a/1a-2a/2a-3a/3b-4a/4a-5a/5a-6a/6a-(7-14)a/a-15a/15b-(16-18)a/a-21a/21a。结论:济南汉族人群HLA和HPA分布具有多态性,与其他地区和种族比较有差异性,应建立本地区血小板捐献者的HLA与HPA基因分型资料库。Objective:To investigate the gene polymorphism distribution of platelet antigen(HPA)1-18,21 and human leukocyte antigen(HLA-A,B)in Han population in Jinan,so as to lay a foundation for establishing the genotyping database of mechanically collected platelet donors in this area.Methods:The HLA-A,B and HPA1-18,21 genotypes of 347 unrelated fixed blood donors were analyzed by fluorescence PCR fusion curve analysis.Results:18 alleles and 34 alleles were detected at HLA-A and B loci respectively.The higher gene frequencies were A^(*)02(0.2781),A^(*)11(0.1686),A^(*)24(0.1470)and B^(*)13(0.1427),B^(*)60(0.0692),B^(*)61(0.0648)and B^(*)62(0.0634).The most common HPA genotypes were:HPA-1 a/1 a-2 a/2 a-3 a/3 b-4 a/4 a-5 a/5 a-6 a/6 A-(7-14)a/a-15 a/15 b-(16-18)a/a-21 a/21 a.Conclusion:The distribution of HLA and HPA in Jinan Han population was polymorphic,which was different from other regions and races.The HLA and HPA genotyping database of platelet donors in this region should be established.

关 键 词：人类血小板抗原 人类白细胞抗原 机采血小板 

分 类 号：R457.1[医药卫生—治疗学]