不同镁剂改性磷酸钙骨水泥的理化与生物性能研究  

Physico-chemical and biological properties of different magnesium modified calcium phosphate bone cements

作　　者：徐海亮 王成文 田方 王致远 师政伟 黄大耿 宋宗让 朱雷[1,2] 贾帅军 贺宝荣 郝定均[1,2] Xu Hailiang;Wang Chengwen;Tian Fang;Wang Zhiyuan;Shi Zhengwei;Huang Dageng;Song Zongrang;Zhu Lei;Jia Shuaijun;He Baorong;Hao Dingjun(Department of Spine Surgery,Honghui Hospital,Xi′an Jiaotong University,Xi′an 710054,China;Shannxi Key Laboratory of Spine Bionic Treatment,Xi′an 710054,China)

机构地区：[1]西安交通大学附属红会医院脊柱外科,西安710054 [2]陕西省脊柱仿生治疗重点实验室,西安710054

出　　处：《中华创伤杂志》2022年第12期1112-1122,共11页Chinese Journal of Trauma

基　　金：国家自然科学基金(81830077);陕西省自然科学基金(2022SF-005);西安市科技计划(2018xasj1004)。

摘　　要：目的探讨不同镁剂改性磷酸钙骨水泥的理化与生物性能。方法将不同镁剂改性磷酸钙骨水泥分为柠檬酸镁组、乳酸镁组、苹果酸镁组、磷酸镁组及甘氨酸镁组,各组又按照其占磷酸钙骨水泥总重量0%、1%、3%及5%的比例添加不同镁剂。检测不同镁剂改性磷酸钙骨水泥的初凝和终凝时间、可注射性、抗溃散性及压缩强度。进一步使用苹果酸镁磷酸钙骨水泥浸提液与第三代成骨细胞进行培养,使用细胞增殖及毒性检测试剂盒(CCK-8)进行生物活性检测;对成骨细胞行碱性磷酸酶(ALP)染色和茜素红S(ARS)染色,观察苹果酸镁磷酸钙骨水泥的成骨活性。结果不同镁剂不同比例添加均导致改性磷酸钙骨水泥的初凝、终凝时间缩短,其中5%苹果酸镁组的终凝时间最短,<40 min,且较同比例其他镁剂改性磷酸钙骨水泥组显著缩短(P均<0.05)。随不同镁剂不同比例添加,骨水泥可注射性逐渐增加,其中5%苹果酸镁组注射性最高,为(87.3±1.9)%,较同比例其他镁剂磷酸钙骨水泥组显著提升(P均<0.05)。骨水泥的抗溃散性随着不同镁剂不同比例添加而下降。柠檬酸镁组、磷酸镁组和甘氨酸镁组不能抵抗去离子水的冲刷。尤以苹果酸镁组的抗溃散性最佳,去离子水冲刷30 min后失重率最大仅为(9.8±2.3)%,优于其余各组(P均<0.05)。乳酸镁组和磷酸镁组的压缩强度较原始磷酸钙骨水泥出现下降,而柠檬酸镁组和苹果酸镁组的压缩强度较原始磷酸钙骨水泥显著提升,其中3%苹果酸镁组的压缩强度最大,达到(6.2±0.2)MPa,较其余各组明显增高(P均<0.05)。筛检出的苹果酸镁磷酸钙骨水泥,其在体外35 d时失重率为(27.0±0.9)%。不同比例苹果酸镁磷酸钙骨水泥体外环境中,镁离子的释放随着苹果酸镁剂量的增加而增加。在35 d内实现稳定的镁离子释放。同时,随着苹果酸镁比例的增加,改性磷酸钙骨水泥对成骨细胞的促增殖�Objective To investigate the physicochemical and biological properties of different magnesium modified calcium phosphate bone cements.Methods The different magnesium modified calcium phosphate bone cements were divided into magnesium citrate,magnesium lactate,magnesium malate,magnesium phosphate and magnesium glycinate groups,each of which was added with different magnesium agents in the proportion of 0%,1%,3%and 5%of the total weight of calcium phosphate bone cements.The initial and final setting time,injectability,anti-collapse performance and compressive strength of different magnesium modified calcium phosphate bone cements were tested.Furthermore,the screened bone cement extracts were used to culture with third generation osteoblasts.Bioactivity assays were performed using the Cell Proliferation and Toxicity Assay Kit(CCK-8).Alkaline phosphatase(ALP)staining and Alizarin Red S(ARS)staining were performed on osteoblasts to observe the osteogenic activity of magnesium malate modified calcium phosphate bone cements.Results The addition of different proportions of different magnesium agents led to the shortening of the initial and final setting time of modified calcium phosphate bone cements.Moreover,the final setting time of 5%magnesium malate modified calcium phosphate bone cements was the shortest(<40 minutes),which was significantly shorter compared with other magnesium agents in the same proportion(all P<0.05).With the addition of different magnesium agents in different proportions,the injectability of bone cements was gradually increased,and the injectability of 5%magnesium malate calcium phosphate bone cements reached the highest for(87.3±1.9)%,which was significantly increased compared with other magnesium agents in the same proportion(all P<0.05).The anti-collapse performance of bone cements was decreased with the addition of different magnesium agents in different proportions.Magnesium citrate,magnesium phosphate and magnesium glycinate modified calcium phosphate bone cements could not resist the flus

关 键 词：骨水泥成形术 磷酸钙 镁 成骨细胞 细胞增殖 

分 类 号：R318.08[医药卫生—生物医学工程]