二补助育汤对着床障碍小鼠内膜胞饮突、趋化因子12及其受体表达的影响  

Effects of Erbu Zhuyu Decoction on the expression of pinocytosis,chemokine 12 and its receptor in endometrium of mice with implantation dysfunction

作　　者：申萌萌 刘雁峰[2] 王铁枫[2] 朱秀娟 郭亚楠 马小娜 SHEN Meng-meng;LIU Yan-feng;WANG Tie-feng;ZHU Xiu-juan;GUO Ya-nan;MA Xiao-na(Beijing University of Chinese Medicine Third Affliated Hospital,Bejing 100029,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京中医药大学东直门医院,北京100700

出　　处：《中华中医药杂志》2022年第12期7054-7058,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81904242)。

摘　　要：目的:观察二补助育汤对着床障碍模型小鼠子宫内膜胞饮突、趋化因子12(CXCL12)和其受体4(CXCR4)表达的影响。方法:24只妊娠ICR小鼠随机为空白组、模型组、补佳乐组、二补助育汤组,每组6只。米非司酮建立着床障碍小鼠模型,各组予相应药物灌胃,妊娠第5天脱颈处死,观察胚胎平均着床位点数及子宫内膜胞饮突表达情况,检测子宫内膜CXCL12、CXCR4mRNA及蛋白表达水平。结果:与空白组比较,模型组小鼠子宫纤细,平均着床位点数下降(P<0.01),胞饮突表达少;CXCL12、CXCR4mRNA表达降低(P<0.01);CXCL12、CXCR4蛋白均降低,其中CXCR4差异有统计学意义(P<0.01)。与模型组比较,补佳乐组和二补助育汤组胞饮突和平均着床位点数表达增多;CXCL12、CXCR4mRNA和蛋白表达增加,其中补佳乐组CXCL12mRNA和二补助育汤组CXCR4蛋白表达较模型组显著增加(P<0.05)。结论:二补助育汤可能通过增加小鼠内膜胞饮突表达,提高CXCL12、CXCR4水平,改善子宫内膜容受性,促进胚胎着床。Objective:To explore the effects of Erbu Zhuyu Decoction on endometrial pinocytosis,CXCL12 and CXCR4 expression in mice with embryo implantation dysfunction.Methods:Twenty-four ICR pregnant mice were randomly divided into blank group,model group,progynova group,Erbu Zhuyu Decoction group,6 mice in each group.Embryo implantation dysfunction model was built with mifepristone.The mice were sacrificed on the 5th day of gestation by necking,the number of embryonic beds and the expression of pinocytosis were observed,the mRNA and protein expressions of CXCL12 and CXCR4 were detected.Results:Compared with the blank group,the uterus of the model group was slender,the number of embryonic beds was decreased(P<0.01),and the expression of pinocytosis was less;The expressions of CXCL12 and CXCR4 mRNA were decreased(P<0.01);The protein expressions of CXCL12 and CXCR4 were decreased,and there was a significant difference in CXCR4 protein(P<0.01).Compared with the model group,pinopodes and embryo implantation site numbers were increased in progynova group and Erbu Zhuyu decoction groups.The mRNA and protein expressions of CXCL12 and CXCR4 increased,among which CXCL12mRNA in progynova group and CXCR4 protein in Erbu Zhuyu Decoction group were significantly higher than model group(P<0.05).Conclusion:Erbu Zhuyu Decoction may improve endometrial receptivity and promote embryo implantation by increasing the expression of pinocytosis and CXCL12 and CXCR4 levels in mouse endometrium.

关 键 词：子宫内膜容受性 胚胎着床障碍 二补助育汤 趋化因子12 趋化因子受体 

分 类 号：R285.5[医药卫生—中药学]