基于肠道微环境探讨针刺联合指压法改善2型糖尿病大鼠菌群群落结构及稳态的研究  

Study on improving flora community structure and homeostasis of type 2 diabetes mellitus rats by acupuncture combined with Zhiya massage based on intestinalmicroenvironment

作　　者：韩怡然 卢泽原 陈邵涛[1] 仲崇文[1] 刚晓超 刘明军[1] HAN Yi-ran;LU Ze-yuan;CHEN Shao-tao;ZHONG Chong-wen;GANG Xiao-chao;LIU Ming-jun(College of Acupuncture and Massage,Changchun University of Chinese Medicine,Changchun 130117,China;Department of Pharmacy,The Second Hospital of Jilin University,Changchun 130041,China)

机构地区：[1]长春中医药大学针灸推拿学院,长春130117 [2]吉林大学第二医院药学部,长春130041

出　　处：《中华中医药杂志》2022年第12期7337-7341,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82174525)。

摘　　要：目的:探讨针刺联合指压法对2型糖尿病(T2DM)大鼠肠道微环境的作用,初步明确针刺联合指压法对菌群群落结构及稳态的影响,及对受损肠道黏膜屏障的修复作用。方法:长期高脂饮食联合小剂量链脲佐菌素诱导建立T2DM大鼠模型。将大鼠随机分为空白组、模型组、针刺组及针刺指压法组。干预4周后,提取大鼠全血、粪便及结肠组织,检测大鼠血液中血糖、白介素-4(IL-4)、肿瘤坏死因子-α(TNF-α)水平;粪便中肠道菌群相对丰度及多样性,以及肠组织中紧密连接蛋白ZO-1、Occludin、Claudin1蛋白表达情况。结果:与模型组比较,针刺指压法组大鼠血糖、葡萄糖耐量实验曲线下面积及血清TNF-α水平均显著降低(P<0.05),血清中IL-4及结肠组织ZO-1、Occludin1蛋白表达水平均显著升高(P<0.05)。针刺联合指压法能够有效改善T2DM大鼠肠道菌群丰度异常及群落多样性减少的情况。结论:针刺联合指压法能够调节葡萄糖代谢异常、改善外周血炎症状态,丰富肠道菌群群落结构及稳态、修复肠道黏膜屏障可能是其治疗T2DM的机制之一。Objective: To investigate the effects of acupuncture combined with Zhiya massage on intestinal micro environment of type 2 diabetes mellitus(T2DM) rats and its related mechanisms. To periluminally clarify the effects of acupuncture combined with Zhiya massage on the community structure and homeostasis of intestinal microflora, as well as the effects on the damaged intestinal mucosal barrier. Methods: T2DM model rats were established by long-term high-fat diet combined with low-dose STZ induction. The rats were randomly divided into control group, model group, acupuncture group and acupuncture combined with Zhiya massage(AM) group. After 4 weeks of intervention, the whole blood, feces, intestinal tissues of rats were extracted. Serum levels of blood glucose, IL-4, TNF-α were detected. The relative abundance and diversity of intestinal flora in feces, and the protein expression of tight junction proteins ZO-1, Occludin and Claudin1 in intestinal tissue were detected.Results: Compared with the model group, the blood glucose and area under curve of glucose tolerance test, and the serum level of TNF-α in MP group were significantly decreased(P<0.05), the level of IL-4 in serum, ZO-1 and Occludin1 protein in the intestinal tissues were significantly increased(P<0.05). Acupuncture combined with Zhiya massage could improve the abnormal abundance of intestinal flora and reduce community diversity in T2DM rats. Conclusion: Acupuncture combined with Zhiya massage can enrich the community structure and homeostasis of intestinal flora and repair the intestinal mucosal barrier, which may be one of the mechanisms why acupuncture combined with Zhiya massage can regulate the abnormal glucose metabolism, improve the inflammatory state of peripheral blood, play a role in the treatment of T2DM.

关 键 词：2型糖尿病 针刺 指压法 肠道菌群 肠道微环境 肠道黏膜屏障 

分 类 号：R245[医药卫生—针灸推拿学]