细胞角蛋白18片段M65和表皮生长因子样结构域蛋白7在结直肠癌患者中的表达及临床价值  

Expression and clinical values of cytokeratin 18 fragment M65 and epidermal growth factor-like domain protein 7 in colorectal cancer patients

作　　者：范国红 杨鸿林 陆赵阳 沈昊 曹季军[2] 凌伟 刘松良[2] 李勇[2] FAN Guohong;YANG Honglin;LU Zhaoyang;SHEN Hao;CAO Jijun;LING Wei;LIU Songliang;LI Yong(Department of Clinical Laboratory,Suzhou Ninth People′s Hospital,Suzhou 215200,Jiangsu;Department of Clinical Laboratory,Taicang First People′s Hospital,Suzhou 215400,Jiangsu,China)

机构地区：[1]苏州市第九人民医院检验科,江苏苏州215200 [2]太仓市第一人民医院检验科,江苏太仓215400

出　　处：《临床检验杂志》2022年第11期826-829,共4页Chinese Journal of Clinical Laboratory Science

基　　金：太仓市医疗卫生应用基础研究项目(TC2021JCYL02)。

摘　　要：目的探讨细胞角蛋白18片段M65和表皮生长因子样结构域蛋白7(EGFL7)在结直肠癌(CRC)患者血清中的表达情况及意义,并分析其与患者病理参数的相关性。方法收集初诊确诊的CRC患者46例、非肠道肿瘤对照患者43例,并收集其基本病理资料包括性别、年龄、瘤体大小、TNM分期以及是否淋巴结转移等。ELISA测定血清中M65、EGFL7的浓度;用Mann-Whitney U检验分析M65、EGFL7在不同患者之间的表达差异以及在不同病理参数的患者间的表达情况差异,受试者工作特征(ROC)曲线用于评价M65、EGFL7以及二者联合检测对CRC的诊断效能。免疫组化法检测CRC肿瘤细胞Ki67表达情况,并将血清M65、EGFL7水平与肿瘤细胞增殖能力进行Pearson相关性分析。结果CRC患者血清M65与EGFL7水平较对照组均显著升高,差异有统计学意义(U=290.5,P<0.0001;U=599,P=0.0012);ROC曲线分析显示M65与EGFL7联合检测筛查CRC的敏感性和特异性分别为86.96%和81.84%,曲线下面积(AUC)为0.9090(95%CI:0.8456~0.9724);不同瘤体大小的CRC患者M65表达水平差异有统计学意义(U=150,P=0.0380),而不同瘤体大小、不同TNM分期以及有无淋巴结转移的CRC患者间EGFL7差异均有统计学意义(U=128,P=0.0091;U=129,P=0.0050以及U=156,P=0.0208);M65、EGFL7表达水平与CRC患者肿瘤细胞增殖能力均未见相关性。结论M65、EGFL7水平在CRC中表达升高,二者联合可有效诊断CRC。EGFL7与患者的临床病理特征的相关性更强,且与患者淋巴结转移与否具有显著的相关性,是CRC疾病预后判断及转移评估的优良标志物。Objective To investigate the expression and clinical values of cytokeratin 18 fragment M65 and epidermal growth factor-like domain protein 7(EGFL7)in serum of the patients with colorectal cancer(CRC)and the correlation with pathological parameters.Methods A total of 46 newly diagnosed CRC patients were enrolled and 43 non-intestinal tumor patients were used as controls,and their basic pathological data including gender,age,tumor size,TNM stage and lymph node metastasis were collected.The concentrations of M65 and EGFL7 in serum were determined by ELISA.The expression differences of M65 and EGFL7 among different patients and the patients with different pathological parameters were analyzed by Mann-Whitney U test.The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficiency of M65,EGFL7 and the combined detection of the two parameters for CRC.Pearson correlation between M65 or EGFL7 levels in serum and the proliferation ability of CRC tumor cells was analyzed.Results The serum levels of M65 and EGFL7 in CRC patients were significantly higher than those in healthy control group(U=290.5,P<0.0001;U=599,P=0.0012).ROC curve analysis showed the combined detection of M65 and EGFL7 presented sensitivity of 86.96%and specificity of 81.84%for CRC screening,and the area under the curve(AUC)was 0.9090(95%CI:0.8456 to 0.9724).The expression level of M65 was significantly correlated with the tumor size of CRC patients(U=150,P=0.0380).However,EGFL7 was significantly correlated with tumor size,TNM stage and lymph node metastasis in CRC patients(U=128,P=0.0091;U=129,P=0.0050 and U=156,P=0.0208,respectively).No significant correlation of the expression levels of M65 and EGFL7 with tumor cell proliferation was found in CRC patients.Conclusion The expression levels of both M65 and EGFL7 were elevated in CRC patients,and the combined determination of the two markers could diagnose CRC effectively.EGFL7 may correlate with multiple clinicopathological characteristics of CRC patients,especially had a signi

关 键 词：细胞角蛋白18片段M65 表皮生长因子样结构域蛋白7 细胞角蛋白 表皮生长因子 结直肠癌 

分 类 号：R446.6[医药卫生—诊断学]