凡纳滨对虾感染WSSV后的荧光定量分析  

Real-time PCR assay for WSSV load in Litopenaeus vannamei

作　　者：钟新英 苏晓茹 张艺娜 张美芳 史晓丽 ZHONG Xin-ying;SU Xiao-ru;ZHANG Yi-na;ZHANG Mei-fang;SHI Xiao-li(College of Life Science,Ningde Normal University,Ningde,Fujian 352100,China)

机构地区：[1]宁德师范学院生命科学学院,福建宁德352100

出　　处：《宁德师范学院学报（自然科学版）》2022年第4期403-407,413,共6页Journal of Ningde Normal University(Natural Science)

基　　金：2020年国家级大学生创新创业训练计划项目;宁德师范学院校级科研项目(2018ZDK14)。

摘　　要：为了解凡纳滨对虾感染白斑综合症病毒(white spot syndrome virus,WSSV)后病毒的增殖变化,采用饲喂法对凡纳滨对虾进行WSSV人工感染,采用real-time PCR方法检测感染初期、高峰期和后期死亡对虾体内的病毒含量.结果表明,随着感染时间的延长,感染初期、高峰期和后期对虾体内WSSV含量范围分别为1.37×105~4.47×10^(6)、1.92×10^(6)~1.30×10^(7)和3.75×10^(6)~1.90×10^(7)copies·ng^(-1),平均病毒含量分别为1.56×10^(6)、7.56×10^(6)和1.02×10^(7)copies·ng^(-1),感染后期对虾体内的WSSV含量显著高于感染初期(P=0.023),且感染后期死亡的对虾对WSSV的耐受力更强.In order to understand the changes in the proliferation of white spot syndrome virus(WSSV)after infection with White spot syndrome virus(WSSV)in Litopenaeus vannamei,a total of 487 shrimp individuals(1.5-2.8 g)of L.vannamei were artificially infected with WSSV.According to the shrimp death curve,shrimp were divided into three phages:early,peak and late phage.The WSSV load in 20 samples of three infection stages were quantified by absolute real-time PCR.The WSSV loads in each stages were 1.37×10^(5)-4.47×10^(6)、1.92×10^(6)-1.30×10^(7)、3.75×10^(6)-1.90×10^(7)copies·g^(-1)DNA,with the average WSSV loads of 1.56×10^(6)、7.56×10^(6)、1.02×10^(7)copies·g^(-1)DNA,respectively.The WSSV loads in late stage were significantly higher than that in early stage(P=0.023).The results also showed that shrimp in late stage had better WSSV resistance.This study will be helpful to reveal the occurrence of WSSV and provide the theoretical value for WSSV resistant breeding of L.vannamei.

关 键 词：凡纳滨对虾 白斑综合症病毒(WSSV) 人工感染 荧光定量 

分 类 号：S917.4[农业科学—水产科学]