硫酸软骨素A、C复配型胶原蛋白基止血抗炎水凝胶的制备及性能研究  

Collagen Based Hydrogel Containing Chondroitin Sulfate A or C with Hemostatic and Anti-inflammatory Potential

作　　者：吴垧妍 李渊丽 周娟 陈敬华 WU Shang-yan;LI Yuan-li;ZHOU Juan;CHEN Jing-hua(School of Biotechnology,Jiangnan University,Wuxi 214122,China;School of Parmaceutical Sciences,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生物工程学院,无锡214122 [2]江南大学药学院,无锡214122

出　　处：《高分子通报》2023年第1期69-81,共13页Polymer Bulletin

基　　金：国家重点研发项目(2021YFC2103100);江苏省自然科学基金青年项目(BK20170203)资助。

摘　　要：胶原蛋白基水凝胶是较为理想的创面修复敷料材料之一,但其对伤口的炎症调节作用仍需要强化。本研究以胶原蛋白为基质材料,复配硫酸软骨素A(CSA)和硫酸软骨素C(CSC)后,通过与醛基化聚乙二醇交联制备水凝胶。通过扫描电镜观察到该水凝胶孔隙致密均一,综合溶胀实验结果显示出良好的吸水率,利于后期应用于伤口时吸收组织渗液。细胞毒性实验及使用激光共聚焦荧光显微镜观察细胞在水凝胶上的生长情况,实验表明水凝胶有利于巨噬细胞和成纤维细胞的增殖。水凝胶的溶血率低于1.5%,同时凝血指数(BCI)明显降低、扫描电镜观察血细胞凝集效果良好。这些结果都表明胶原蛋白基硫酸软骨素复合水凝胶具备作为止血抗炎敷料的潜力。The primary consideration in wound treatment is hemostasis and regulating inflammation,so the development of hemostatic and anti-inflammatory dressings is of great significance.In this study,two kinds of hydrogels based on collagen were prepared and their hemostatic and anti-inflammatory effects were tested.Collagen was compounded with chondroitin sulfate A(CSA)and chondroitin sulfate C(CSC),which have anti-inflammatory effects,respectively,and the resulting complex was chemically cross-linked with aldehyde benzoic acid modified polyethylene glycol 6000(APG6000)to form hydrogels.The hydrogel had a sudden release of chondroitin sulfate A or chondroitin sulfate C,which had the potential of rapid anti-inflammatory when applied to skin wounds.The pores of the hydrogels obversed by the scanning electron microscope(SEM)were compact and uniform.The results of swelling experiment showed favorable water absorption.Both of above properties were beneficial to absorb tissue exudation when applying hydrogels to wound.The results of MTT and living cell staining experiments reflected that the two hydrogels contributed to proliferation of macrophages and fibroblasts.The hemolysis rate of the hydrogels was lower than 1.5%,the blood clotting index(BCI)decreased significantly,and the hemagglutination effect was positive.These results indicated that the two hydrogels have the potential as hemostatic and anti-inflammatory dressings.

关 键 词：硫酸软骨素 胶原蛋白 止血 抗炎 水凝胶 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]