七十味珍珠丸干预脑梗死模型大鼠软脑膜微循环及胶质瘢痕的变化  

Effect of Ratnasampil on pia microcirculation and glial scar in a rat model of cerebral infarction

作　　者：马辉 孙正启[1] 李岩松 Ma Hui;Sun Zhengqi;Li Yansong(Department of Human Morphology,Xizang Minzu University School of Medicine,Xianyang 712000,Shaanxi Province,China)

机构地区：[1]西藏民族大学医学院人体形态学教研室,陕西省咸阳市712000

出　　处：《中国组织工程研究》2023年第28期4462-4467,共6页Chinese Journal of Tissue Engineering Research

基　　金：西藏自治区自然科学基金项目(XZ202101ZR0091G),项目负责人:马辉。

摘　　要：背景:改善脑梗死微脑膜循环、抑制瘢痕形成可有效治疗脑梗死疾病,因此寻找安全有效的药物来改善脑微循环及瘢痕形成至关重要。目的:探讨七十味珍珠丸对脑梗死大鼠软脑膜微循环、Janus激酶2(Janus Kinase2,JAK2)/转录激活因子3(signal transducer and activator transcription3,STAT3)蛋白表达及胶质瘢痕的影响。方法:于95只雄性SD大鼠中随机抽取15只作为健康组,剩余大鼠建立脑梗死模型,将模型大鼠随机分为模型组、中药低、中、高剂量组以及尼莫地平组,在建模过程中意外死亡5只,剩余75只建模成功,每组15只。中药低、中、高剂量组分别于造模前25 min给予16.67,33.34,66.68 g/kg七十味珍珠丸混悬液灌胃1次,尼莫地平组于造模前25 min给予30 mg/kg尼莫地平片灌胃1次。建模后运用MCIP微循环图像处理系统检测脑血流速度,苏木精-伊红染色观察脑组织病理学形态,RT-PCR检测JAK2、STAT3、神经蛋白聚糖、胶质纤维酸性蛋白基因水平,TUNEL技术检测脑组织细胞凋亡情况,免疫印迹检测JAK2、STAT3、p-STAT3、神经蛋白聚糖、胶质纤维酸性蛋白的蛋白表达。结果与结论:(1)与健康组相比,模型组不同时间点脑血流速度降低,JAK2、STAT3、p-STAT3、神经蛋白聚糖、胶质纤维酸性蛋白、神经元细胞凋亡率升高(P<0.05);(2)与模型组相比,中药低剂量组不同时间点脑血流速度升高,JAK2、STAT3、磷酸化转录激活因子3、神经蛋白聚糖、胶质纤维酸性蛋白、神经元细胞凋亡率降低(P<0.05);(3)与中药中剂量组相比,中药高剂量组与尼莫地平组不同时间点脑血流速度升高,JAK2、STAT3、p-STAT3、神经蛋白聚糖、胶质纤维酸性蛋白、神经元细胞凋亡率降低(P<0.05);(4)与中药高剂量组相比,尼莫地平组不同时间点脑血流速度降低,JAK2、STAT3、p-STAT3、神经蛋白聚糖、胶质纤维酸性蛋白、神经元细胞凋亡率升高(P<0.05);(5)健康组大鼠脑皮质�BACKGROUND:Improving cerebral infarction micromeningeal circulation and inhibiting scar formation can effectively treat cerebral infarction.Therefore,it is very important to develop safe and effective drugs to improve cerebral microcirculation and scar formation.OBJECTIVE:To investigate the effects of Ratnasampil on pia meningeal microcirculation,Janus kinase 2/signal transducer and activator transcription 3 protein expression and glial scar in rats with cerebral infarction.METHODS:The 15 of 95 male Sprague-Dawley rats were randomly selected as healthy group and the remaining rats used to establish cerebral infarction models.Five rats died accidentally during the modeling process and the remaining rats were successfully modeled.Model rats were divided into model group,low-dose,medium-dose and high-dose groups of Traditional Chinese medicine and nimodipine group with 15 rats in each group.Low-,medium-and highdose groups were given 16.67,33.34 and 66.68 g/kg Ratnasampil suspension once by intragastric administration respectively at 25 minutes before modeling.The nimodipine group was given 30 mg/kg nimodipine tablet once by intragastric administration at 25 minutes before modeling.MCIP microcirculation image processing system was used to detect cerebral blood flow velocity.Hematoxylin-eosin staining was used to observe brain histopathological morphology.Real-time fluorescence quantitative PCR was used to detect gene levels of Janus kinase 2,signal transducer and activator transcription 3,neurocan and glial fibrillary acidic protein.In situ terminal transferase labeling technique was used to measure cell apoptosis.Western blot was used to detect the protein expression of Janus kinase 2,signal transducer and activator transcription 3,phosphorylated signal transducer and activator transcription 3,neurocan and glial fibrillary acidic protein.RESULTS AND CONCLUSION:Compared with the healthy group,the nimodipine group showed reduced cerebral blood flow velocity,increased expression of Janus kinase 2,signal transducer and

关 键 词：七十味珍珠丸 脑梗死大鼠 软脑膜微循环 JAK2/STAT3 胶质瘢痕 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R255.2