骨疏康干预Beagle犬磨牙压低过程中转化生长因子β1及β-连环蛋白的表达  

Effects of Gushukang on transforming growth factor beta1 and beta-catenin during molar intrusion in Beagle dogs

作　　者：万哲[1] 杜军[1] 胡杨[2] Wan Zhe;Du Jun;Hu Yang(Department of Stomatology,Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830000,Xinjiang Uygur Autonomous Region,China;Department of Implant and Prosthodontics,Stomatological Hospital,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学附属中医医院口腔科,新疆维吾尔自治区乌鲁木齐市830000 [2]新疆医科大学第一附属医院口腔医院口腔种植修复科,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《中国组织工程研究》2023年第28期4502-4506,共5页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金面上项目(2019D01C185),项目负责人:万哲。

摘　　要：背景:骨疏康可以有效促进破骨细胞的凋亡、提高骨密度和骨矿含量、抑制骨吸收。但骨疏康在牙根吸收方面作用的研究匮乏,其调控作用机制尚不清楚。目的:探讨骨疏康在Beagle犬正畸牙根吸收过程中的治疗效果及其对转化生长因子β1、β-连环蛋白表达的影响。方法:将16只Beagle犬随机分为正常对照组(n=4)、蒸馏水组(n=6)与骨疏康组(n=6),正常对照组不进行任何干预,蒸馏水组与骨疏康组建立上颌第一磨牙压低模型,造模后分别每天灌胃蒸馏水与骨疏康(2.1 g/kg)。干预6,9,12周,收集上颌第一磨牙牙根和牙周组织,采用苏木精-伊红染色观察病理变化,免疫组化检测转化生长因子β1、β-连环蛋白的表达水平。结果与结论:(1)第一磨牙随时间推移有明显压低现象出现,在第12周效果最为明显;(2)苏木精-伊红染色显示,蒸馏水组磨牙组织随着加力时间周期的不断延长,破骨细胞数量呈上升趋势,骨吸收现象不断加重;骨疏康组磨牙的根吸收程度轻于蒸馏水组,骨修复能力和牙周组织的改建能力优于蒸馏水组;(3)蒸馏水组磨牙组织转化生长因子β1和β-连环蛋白的表达量显著低于正常对照组(P<0.05);骨疏康组磨牙组织中转化生长因子β1和β-连环蛋白的表达水平随时间推移呈上升趋势,在9周和12周的表达水平显著高于蒸馏水组(P<0.05);(4)结果表明,骨疏康可能通过激活调节转化生长因子β1和β-连环蛋白,促进Beagle犬磨牙骨组织的成骨分化。BACKGROUND:Gushukang can effectively promote apoptosis of osteoclasts,increase bone mineral density and bone mineral content,and inhibit bone resorption.However,studies on root resorption are scarce and its regulatory mechanism remains unclear.OBJECTIVE:To investigate the effect of Gushukong on root resorption and expression of transfo rming growth factor-β1 and β-catenin in Beagle dogs.METHODS:Sixteen Beagle dogs were randomly divided into normal control group(n=4),distilled water group(n=6),and Gushukang group(n=6).Normal control group did not do any treatment.Distilled water group and Gushukang group established Maxillary first molar intrusion models were established in the distilled water and Gushukang groups.After modeling,distilled water and Gushukang groups were intragastrically given distilled water and Gushukang at a dose of 2.1 g/kg body mass respectively.After 6,9,and 12 weeks of intervention,Beagle dogs were anesthetized to collect root and periodontal tissues of the maxillary first molars.The pathological changes were observed by hematoxylin-eosin staining and the expression levels of transforming growth factor-β1 and β-catenin were detected by immunohistochemistry.RESULTS AND CONCLUSION:The maxillary first molars were obviously intruded over time,especially at the 12thweek.The results of hematoxylin-eosin staining revealed that the number of osteoclasts in molar tissue increased in the distilled water group with the prolongation of loading,accompanied by aggravated bone resorption.The Gushukang group had less resorption of molar root and better ability of bone repair and periodontal tissue remodeling than the distilled water group.The expression levels of transfo rming growth factor-β1 andβ-catenin in the distilled water group were significantly lower than those in the normal control group(P<0.05),while the expression levels of transforming growth factor-β1 and β-catenin in molar tissue of the Gushukang group increased with time,which were significantly higher than those in the distilled wat

关 键 词：根吸收 骨疏康 转化生长因子Β1 Β-连环蛋白 BEAGLE犬 正畸牙 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R783.5