补肾壮骨方干预去卵巢骨质疏松模型大鼠骨代谢及骨密度的变化  

Effect of Bushen Zhuanggu Fang on bone metabolism and bone mineral density in rats with ovariectomized osteoporosis

作　　者：苏辉 闫炳翰 王若冲 薛海鹏[3] 谭国庆[3] 徐展望[1,3] Su Hui;Yan Binghan;Wang Ruochong;Xue Haipeng;Tan Guoqing;Xu Zhanwang(Shandong University of Chinese Medicine,Jinan 250355,Shandong Province,China;Beijing University of Chinese Medicine,Beijing 100000,China;Affiliated Hospital of Shandong University of Chinese Medicine,Shandong 250014,Shandong Province,China)

机构地区：[1]山东中医药大学,山东省济南市250355 [2]北京中医药大学,北京市100000 [3]山东中医药大学附属医院,山东省济南市250014

出　　处：《中国组织工程研究》2023年第28期4507-4512,共6页Chinese Journal of Tissue Engineering Research

基　　金：山东省自然科学基金面上项目(ZR2020MH362),项目负责人:谭国庆;山东省自然科学基金项目(ZR2020KH011),项目负责人:徐展望;国家自然科学基金面上项目(82174410),项目负责人:徐展望。

摘　　要：背景:中医药以其良好的治疗效果及安全性越来越多的应用于骨质疏松症的治疗中,补肾壮骨方目前作为山东中医药大学附属医院协定方被广泛应用于临床中,获得了极好的治疗效果。目的:评价补肾壮骨方对去卵巢骨质疏松大鼠骨代谢及骨密度的影响。方法:60只SD大鼠随机分为6组,分别为假手术组、模型组、阿仑膦酸钠组、补肾壮骨方高、中、低剂量组,每组10只,后5组制备去卵巢骨质疏松症模型,假手术组只切除卵巢周围脂肪组织。造模8周后,补肾壮骨方高、中、低剂量组分别予以2.34,1.17,0.58 g/(kg·d)补肾壮骨方,阿仑膦酸钠组予阿仑膦酸钠1 mg/(kg·d),假手术组及模型组给予等体积的生理盐水,1次/d灌胃。连续灌胃12周后检测各组大鼠血清碱性磷酸酶、Runt相关转录因子2及核因子κB受体活化因子配体水平;取右侧股骨观察骨密度及骨微结构;苏木精-伊红染色观察组织病理改变;免疫组化法检测骨组织Wnt1、骨形态发生蛋白2蛋白表达。结果与结论:(1)造模8周后,与假手术组对比,模型组大鼠骨密度、骨小梁数明显降低(P<0.05),提示造模成功;(2)与模型组大鼠对比,补肾壮骨方低、中、高剂量组及阿仑膦酸钠组骨密度、大鼠骨体积比、骨表面积/组织体积的比值、骨小梁数明显增加(P<0.01),骨小梁分离度显著降低(P<0.01),其中阿仑膦酸钠组的改善作用优于补肾壮骨方组;苏木精-伊红染色结果显示,与模型组大鼠相比,补肾壮骨方低、中、高剂量组与阿仑膦酸钠组骨小梁数量增多,连续性增加,说明补肾壮骨方在去卵巢大鼠骨微结构上可明显改善骨骼状态;(3)补肾壮骨方低、中、高剂量组大鼠血清中Runt相关转录因子2水平高于模型组(P<0.01),碱性磷酸酶、核因子κB受体活化因子配体水平低于模型组(P<0.01),其中阿仑膦酸钠组的改善作用优于补肾壮骨方组;免疫组化结果显示,补肾壮BACKGROUND:Chinese medicine with its good treatment effect and safety is increasingly applied to the treatment of osteopo rosis.Bushen Zhuanggu Fong is currently widely used in clinical practice as an agreement of the Affiliated Hospital of Shandong University of Chinese Medicine,and has obtained excellent treatment results.OBJECTIVE:To evaluate the effects of Bushen Zhuanggu Fang on bone metabolism and bone density in a rat model of ovariectomized osteoporosis.METHODS:Sixty Sprague-Dawley rats were randomly grouped into sham group,model group,alendronate sodium group,high-,middle-,and low-dose Bushen Zhuanggu Fang groups(n=10 per group).Rat models of ovariectomized osteopo rosis were made in the latter five groups.The sham group received no intervention except for the removal of periovarian adipose tissue.After 8 wee ks of modeling,high-,middle-and low-dose Bushen Zhuanggu Fang groups were given 2.34,1.17,0.58 g/kg per day,respectively,the alendronate sodium group was given 1 mg/kg per day,and sham operation group and model group were intragastrically given the same volume of normal saline,once a day.The serum levels of alkaline phosphatase,RUNT-related transcri ption factor 2 and receptor activator of nuclear factor-κB ligand were detected after 12 weeks of continuous gavage.The bone mineral density and microstructure of the right femur were observed.Hematoxylin-eosin staining was used to observe histopathological changes.The expressions of Wnt1 and bone morphogenetic protein 2 were immunohistochemically detected.RESULTS AND CONCLUSION:Compared with the sham group,the bone mineral density and bone trabecular number of the model group were significantly reduced at 8 weeks after modeling(P<0.05),indicating the successful modeling.Compared with the model group,the bone mineral density,bone volume ratio,bone surfa ce area/tissue volume ratio,and the number of bone trabeculae were significantly increased,while trabecular separation was significantly reduced in the low-,middle-,and high-dose Bushen Zhuanggu Fang gro

关 键 词：补肾壮骨方 去卵巢 骨质疏松 骨代谢 骨密度 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R274