互花米草入侵背景下增氮对滨海湿地土壤可培养细菌和真菌群落组成及多样性的影响  

Effects of Nitrogen Input Increase on Composition and Diversity of Culturable Soil Bacterial and Fungal Communities under Spartina alterniflora Invasion in Coastal Wetland

作　　者：王欢欢 王银华 庞金玲 张慧敏 王佳宁[1,2] 佟雨徽 郭宏宇 Wang Huanhuan;Wang Yinhua;Pang Jinling;Zhang Huimin;Wang Jianing;Tong Yuhui;Guo Hongyu(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《南开大学学报（自然科学版）》2022年第5期96-105,共10页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(32001179,31300357);天津自然科学基金(20JCZDJC00220)。

摘　　要：为探究互花米草入侵背景下增氮对滨海湿地土壤微生物群落组成和多样性的影响,在天津滨海湿地选取互花米草入侵程度不同样地进行模拟增氮实验,研究不同实验处理条件下土壤可培养细菌和真菌数量、群落组成和多样性差异,及其与土壤理化性质间相关关系.结果表明,滨海湿地土壤细菌和真菌数量和多样性均随互花米草入侵程度的增加先升高后降低;细菌数量和多样性随氮输入水平增加而升高,而真菌数量和多样性随氮输入水平增加而降低.相关性分析和冗余分析显示,互花米草入侵和氮输入增加主要通过改变土壤氮含量、pH值和有效磷含量影响滨海湿地土壤微生物数量、群落组成和多样性.研究表明,氮输入增加将在一定程度上缓解互花米草入侵对滨海湿地土壤可培养细菌群落的负面作用,而增强互花米草入侵对滨海湿地土壤可培养真菌群落的不利影响.In order to explore the effects of nitrogen input increase on the composition and diversity of soil microbial communities in coastal wetland under Spartina alterniflora invasion,simulated nitrogen addition experiments were conducted in Tianjin coastal wetland.The differences in the quantities,community composition and diversity of soil culturable bacteria and fungi,and their relationships with soil factors were analyzed.The results showed that the quantities and diversity of bacteria and fungi increased and then decreased with the increase of S.alterniflora invasion.The quantities and diversity of bacteria increased with the increase of nitrogen input level,while the quantities and diversity of fungi decreased with the increase of nitrogen input level.The results of correlation analyses and RDA showed that the invasion of S.alterniflora and the increase of nitrogen input level affected the quantities,community composition and diversity of soil microorganisms in coastal wetland mainly by altering soil nitrogen content,pH,and available phosphorus content.This study indicated that increased nitrogen input would alleviate the negative effects of S.alterniflora invasion on soil culturable bacterial communities,but would strengthen the negative effects of S.alterniflora invasion on soil culturable fungal communities in coastal wetland to some extent.

关 键 词：互花米草 氮输入 滨海湿地 微生物群落 多样性 

分 类 号：Q938.1[生物学—微生物学]