心房颤动患者血清NLRC4/Caspase-1表达及临床意义  

Expression and clinical significance of serum NLRC4/Caspase-1 in patients with atrial fibrillation

作　　者：邹仕英 王聪 严冲 唐碧[1] 康品方[1,2] 冷俊杰 张恒[1] ZOU Shiying;WANG Cong;YAN Chong;TANG Bi;KANG Pinfang;LENG Junjie;ZHANG Heng(Department of Cardiology,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui,233000,China;Key Laboratory of Basic and Clinical Medicine of Cardiovascular and Cerebrovascular Diseases,the First Affiliated Hospital of Bengbu Medical College)

机构地区：[1]蚌埠医学院第一附属医院心血管科,安徽蚌埠233000 [2]蚌埠医学院第一附属医院心脑血管疾病基础与临床重点实验室

出　　处：《临床心血管病杂志》2022年第12期988-993,共6页Journal of Clinical Cardiology

基　　金：国家自然科学基金(No:81970313);安徽省自然科学基金(No:1908085QH353);蚌埠医学院研究生创新课题(No:Byycx21060)。

摘　　要：目的通过检测心房颤动(房颤)患者血清中NLR家族含CARD结构蛋白4(NLRC4)和半胱氨酸天冬氨酸特异蛋白酶1(Caspase-1)的表达水平变化,探讨二者在房颤发展中的规律及临床意义。方法采用横断面研究方法,选取2020年12月—2021年12月在我院接受治疗的房颤患者200例,其中阵发性房颤(paroxysmal atrial fibrillation,PaAF)组61例,持续性房颤(persistent atrial fibrillation,PeAF)组71例,永久性房颤(permanent atrial fibrillation,PmAF)组68例。并选取90例同时期体检窦性心律(sinus rhythm,SR)者为对照组。ELISA和Western blot法分别测定血清中NLRC4、半胱氨酸天冬氨酸特异性蛋白酶1(Caspase-1)水平及通路蛋白表达情况,并分别与已测得的左心房内径(left atrial diameter,LAD)进行相关性分析。结果与对照组比较,PaAF组、PeAF组和PmAF组NLRC4、Caspase-1表达水平升高(P<0.01)。与PeAF组比较,PmAF组NLRC4、Caspase-1表达水平升高(P<0.01)。与PaAF组比较,PeAF组和PmAF组NLRC4、Caspase-1表达水平升高(P<0.01)。Western blot检测NLRC4/Caspase-1通路中各蛋白表达量,均存在上调趋势。Pearson法对NLRC4、Caspase-1与LAD进行相关性分析,结果显示在所有试验组中NLRC4浓度与Caspase-1、LAD呈正相关(r=0.540、0.516,P<0.001);Caspase-1与LAD呈正相关(r=0.363,P<0.001)。结论NLRC4与Caspase-1在房颤患者血清表达水平明显升高,且与LAD有一定的相关性,NLRC4/Caspase-1的激活与表达可能与房颤的发生有关。Objective To investigate the regulation and clinical significance of NLRC4 and Caspase-1 in the development of atrial fibrillation by detecting the expression levels of NLRC4 and Caspase-1 in the serum of atrial fibrillation patients.Methods A cross-sectional study was conducted to select 200 ATRIAL FIBRILLATION patients treated in our hospital from December 2020 to December 2021,including 61 patients in the paroxysmal atrial fibrillation(PaAF)group.There were 71 patients in the persistent atrial fibrillation(PeAF)group and 68 patients in the permanent atrial fibrillation(PmAF)group.A total of 90 patients with sinus rhythm(SR)were selected as the control group.The levels of NLRC4 and caspase-1 and the expression of pathway proteins in serum were measured by ELISA and Western blot,and the correlation between NLRC4 and LAD was analyzed.Results Compared with the control group,the expression levels of NLRC4 and Caspase-1 in PaAF,PeAF,and PmAF groups were significantly increased(P<0.01).Compared with the PeAF group,the expression levels of NLRC4 and Caspase-1 in the PmAF group were significantly increased(P<0.01).Compared with the PaAF group,the expression levels of NLRC4 and caspase-1 in PeAF and PmAF groups were significantly increased(P<0.01).The expression levels of NLRC4/Caspase-1 proteins were up-regulated by Western blot.The Pearson method was used to analyze the correlation between NLRC4,Caspase-1,and LAD.The results showed that the concentration of NLRC4 was positively correlated with Caspase-1 and LAD in all experimental groups(r=0.540,0.516,P<0.001),Caspase-1 was positively correlated with LAD(r=0.363,P<0.001).Conclusion The expression levels of NLRC4 and Caspase-1 in the serum of atrial fibrillation patients are significantly increased,and there is a certain correlation with LAD.The activation and expression of NLRC4/Caspase-1 may be related to the occurrence of atrial fibrillation.

关 键 词：心房颤动 NLRC4 半胱氨酸天冬氨酸特异性蛋白酶1 左心房内径 

分 类 号：R541.7[医药卫生—心血管疾病]