酸柏栀油软胶囊促智作用GPR40的机制研究  

Study on the Mechanism of Soft Capsules of Compound Oil of Jujube,Arboruitae and Gardenia Improving the Intelligence of GPR40

作　　者：苗梅静[1] 苏鹏飞 刘生梅[1] MIAO Meijing;SU Pengfei;LIU Shengmei(Medical School of Yan′an University,Yan′an 716000,China;Yan′an University)

机构地区：[1]延安大学医学院,陕西延安716000 [2]延安大学

出　　处：《西部中医药》2022年第12期35-39,共5页Western Journal of Traditional Chinese Medicine

基　　金：陕西省教育厅专项科研计划(16JK1862);延安大学校级科研项目(YDK2015-61)。

摘　　要：目的:观察酸柏栀油软胶囊(compound oil of jujube,arboruitae,and gardenia,COJAG)对记忆获得障碍模型小鼠的促智作用,分析COJAG促智作用GPR40的具体机制。方法:选购昆明种雄性小鼠152只,适应性喂养1周后,随机选择24只为空白对照组,其他128只均用于记忆获得障碍模型的建立,128只小鼠建模成功120只,均衡随机分为5组,每组24只,即模型组,脑复康组,COJAG低、中、高剂量组。各组均按设定剂量灌胃给药,每日1次,均连续灌胃40天。结束后经Morris水迷宫实验检测小鼠行为学,免疫荧光组化检测GPR40在中枢神经系统中的表达和分布。结果:第1~5天,模型组小鼠寻找平台潜伏期及寻找平台路径高于其他组(P<0.05);用药后,COJAG组、脑复康组寻找平台潜伏期及寻找平台路径均较模型组降低(P<0.05);COJAG高剂量组寻找平台潜伏期及寻找平台路径最短,与低剂量组比较差异有统计学意义(P<0.05),但与中剂量组比较差异无统计学意义(P>0.05);较空白对照组,模型组小鼠第Ⅲ象限停留时间延长、第Ⅲ象限路程百分比降低、穿台次数减少(P<0.05);用药后,COJAG组、脑复康组小鼠第Ⅲ象限停留时间缩短、第Ⅲ象限路程百分比升高、穿台次数增加(P<0.05);COJAG高剂量组第Ⅲ象限停留时间最短、第Ⅲ象限路程百分比最高、穿台次数最多,与低剂量组比较差异有统计学意义(P<0.05),但与中剂量组比较差异无统计学意义(P>0.05)。较其他组,对照组小鼠GRP40表达最高,后由高至低依次为脑复康组、COJAG高剂量组、COJAG中剂量组、COJAG低剂量组,模型组表达最低。结论:使用酸柏栀油软胶囊可使脑组织GPR40升高,可以促进神经前体细胞增殖与神经元细胞突触增长,这可能是酸柏栀油软胶囊促记忆障碍小鼠学习能力提高的主要机制。Objective:To observe the effects of soft capsules of compound oil of jujube,arboruitae and gardenia(COJAG)on the intelligence of memory acquisition impairment mice models,and analyze the specific mechanism of COJAG improving the brain of GPR40.Methods:All 152 Kunming male mice were chosen,after one week of adaptive feeding,24 ones were selected as blank control group,the remaining 128 mice were established into memory acquisition impairment models,and 120 successful mice models were randomized into five groups,24 ones in each group,that is,the model group,Naofukang group,low,moderate and high dosages groups of COJAG.All the groups were drenched with the medicine in the set dose,once each day,for 40 days consecutively.By the end of the experiment,Morris water mase was used to detect the mice’s behaviors,immunofluorescence and immunohistochemistry were applied to detect the expressions and distribution of GPR40 in central nervous system.Results:From the first day to the fifth day,the latency and path of finding platform in the model group were higher than these in the other groups(P<0.05);after the medication,the latency and path of finding platform in COJAG group and Naofukang group were reduced compared with these of the model group(P<0.05);the latency and path of finding platform in high dose group of COJAG were the shortest,the difference was statistically significant compared with low dose group(P<0.05),the difference had no statistical meaning compared with moderate dose group(P>0.05);Compared with blank control group,the length of stay at the third quadrant prolonged,the percentage of the journey at the third quadrant lowered and the times of crossing the platform reduced in the mice of the model group(P<0.05);after the medication,the length of stay at the third quadrant shortened,the length of stay at the third quadrant increased and the times of crossing the platform added in COJAG group and Naofukang group(P<0.05);in high dose group of COJAG,the length of stay at the third quadrant was the shortest,the

关 键 词：学习记忆障碍 酸柏栀油软胶囊 行为学 GPR40 小鼠 动物实验 

分 类 号：R285[医药卫生—中药学]