血清抗Jo-1抗体、抗dsDNA抗体与复发性流产的相关性  

Correlation between the levels of serum anti Jo-1 antibody and anti-double stranded DNA antibody of women and their recurrent abortion

作　　者：何末琴 邵瑾 HE Moqin;SHAO Jin(Changxing Branch of Children's Hospital,Zhejiang University School of Medicine(Changxing County Maternal and Child Health Care Hospital),Changxing,Zhejiang Province,313100)

机构地区：[1]浙江大学医学院附属儿童医院长兴分院(长兴县妇幼保健院),313100

出　　处：《中国计划生育学杂志》2023年第1期159-162,共4页Chinese Journal of Family Planning

基　　金：湖州市科学技术局项目(2017GY59)。

摘　　要：目的:检测复发性流产患者血清抗双链DNA抗体(dsDNA)、抗Jo-1抗体的表达并探究其与复发性流产关系。方法:选取2019年1月-2020年2月本院收治的复发性流产患者100例为复发性流产组,无不良孕产史且已正常生育的女性100例作为对照组。采用酶联免疫吸附法检测两组血清抗Jo-1抗体、抗dsDNA抗体及抗心磷脂抗体(ACA)表达水平及对复发性流产的诊断价值,logistic回归分析复发性流产发生的相关影响因素。结果:复发性流产组血清抗Jo-1抗体(23.0%)、抗dsDNA抗体(17.0%)、ACA阳性(22.0%)表达率均高于对照组(5.0%、4.0%、8.0%)(均P<0.05);复发性流产组中抗Jo-1抗体和抗dsDNA抗体联合检测阳性率较抗Jo-1抗体、抗dsDNA抗体、ACA单独检测及抗Jo-1抗体和ACA抗体联合检测和抗dsDNA抗体和ACA抗体联合检测阳性率高(P<0.05)。受试者工作特征曲线分析显示,抗Jo-1抗体、抗dsDNA抗体及二者联合诊断复发性流产的曲线下面积分别为0.590、0.570、0.660,敏感度分别为95.0%、97.0%、94.0%,特异度分别为23.0%、17.0%、38.0%。血清抗Jo-1抗体阳性表达、抗dsDNA抗体阳性表达、ACA阳性表达是影响复发性流产发生的独立危险因素。结论:复发性流产患者血清中抗Jo-1抗体、抗dsDNA抗体呈高表达,二者异常高表达是复发性流产发生的危险因素,可作为复发性流产的早期诊断生物学指标。Objective:To detect the expressions of anti-double stranded DNA(dsDNA)antibody and anti Jo-1 antibody of women with recurrent abortion,and to explore the correlation between the expression levels of serum anti Jo-1 antibody and anti dsDNA antibody of these women and their recurrent abortion.Methods:A total of 100 women with recurrent abortion were selected in study group,and 100 women without adverse pregnancy history and with normal childbirth history were selected in control group from January 2019 to February 2020.The expression levels of anti Jo-1 antibody,anti dsDNA antibody,and anticardiolipin antibody(ACA)of the women in the two groups were detected by enzyme-linked immunosorbent assay(ELISA).The diagnostic value of anti Jo-1 antibody,anti dsDNA antibody,and ACA of the women for their recurrent abortion was analyzed.Logistic regression analysis was used to analyze the related factors affecting the recurrent abortion of the women.Results:The expression rates of anti-JO-1 antibody(23.0%),anti DSDNA antibody(17.0%),and ACA positive(22.0%)of the women in the study group were significantly higher than those(5.0%,4.0%,and 8.0%)of the women in the control group(all P<0.05).In the study group,the positive rate of anti-JO-1 antibody combined with anti-DSDNA antibody detections was significantly higher than that of the anti-JO-1 antibody detection,anti-DSDNA antibody detection,or ACA detection alone,or the anti-JO-1 antibody combined with ACA antibody detections,or anti-DSDNA antibody combined with ACA antibody detections(P<0.05).Receiver operating characteristic(ROC)curve analysis showed that the areas under curves of the anti-JO-1antibody level,the anti-DSDNA antibody level,and the combination of anti-JO-1antibody level and anti-DSDNA antibody level of the women for diagnosing their recurrent abortion were 0.590,0.570,and 0.660,respectively,the sensitivity of which were 95.0%,97.0%,and 94.0%,respectively,and the specificity of which were 23.0%,17.0%,and 38.0%,respectively.The serum anti-JO-1antibody positive expre

关 键 词：复发性流产 抗JO-1抗体 抗双链DNA抗体 诊断价值 影响因素 

分 类 号：R714.21[医药卫生—妇产科学]