以肠道菌群为靶点的HIIT干预效果与NPY基因多态性研究  

Study on Gut Microbiota-targeted HIIT Intervention and NPY Gene Polymorphism

作　　者：聂晶[1] 刘才响 周多奇 徐山茸 杨慧林[1] NIE Jing;LIU Caixiang;ZHOU Duoqi;XU Shanrong;YANG Huilin(Jiangxi Normal University,Nanchang 330022,Jiangxi China;Anqing Normal University,Anqing 246133,Anhui China)

机构地区：[1]江西师范大学,江西南昌330022 [2]安庆师范大学,安徽安庆246133

出　　处：《北京体育大学学报》2022年第10期61-74,共14页Journal of Beijing Sport University

基　　金：国家重点研发计划“运动健康促进效果个体差异的生物学机制与健身指导方案”(项目编号:2018YFC2000602)。

摘　　要：目的:研究高强度间歇训练(HIIT)对18~24岁汉族人群肠道菌群、体成分的影响,及与NPY基因rs16123多态性的关联,以期将肠道菌群为靶点为不同遗传特征人群提供个体化的运动健身指导方案。方法:选取55名健康汉族大学生为研究对象,进行为期12周HIIT干预,每周训练3次,每次28 min,跑走交替,强度分别为80%~90%、50%~55%V·O_(2)max。干预前抽取静脉血、收集粪便并测试体成分;干预后再次收集粪便及测试体成分相关指标。提取粪便DNA经扩增后在Illumina HiSeq平台进行高通量测序分析肠道菌群。NPY基因rs16123基因分型采用Illumina CGA基因芯片完成。结果:(1)NPY基因rs16123多态性各基因型分布频率均符合H-W平衡。(2)12周的HIIT干预后,NPY基因rs16123多态性各基因型者骨骼肌质量、骨骼肌质量百分比、去脂体重均显著性增加,体脂百分比显著降低(P<0.05),且NPY基因rs16123多态性与体脂百分比变化率有关联,即AA基因型体脂百分比降低幅度显著高于AG+GG基因型。(3)12周的HIIT干预后,AA基因型者OTU数目增加,且α多样性中ACE指数、Chao1指数均显著上升(P<0.05);在菌纲水平分类中Gammaproteobacteria相对丰度显著下降(P<0.05);在菌目水平分类中Enterobacteriales、Pasteurellales相对丰度显著下降(P<0.05);在菌科水平分类中Enterobacteriaceae、Pasteurellaceae、Peptostreptococcaceae相对丰度均显著下降,Lachnospiraceae相对丰度显著增加(P<0.05);在菌属水平分类中Lactobacillus相对丰度显著增加(P<0.05)。而AG+GG基因型者OTU数目有增加,α多样性各指数无显著性差异(P>0.05);在菌目分类水平中Actinomycetales相对丰度显著降低(P<0.05);在菌科水平中Comamonadaceae相对丰度显著降低(P<0.05)。(4)12周HIIT干预前,AA基因型者OTU数目高于AG+GG基因型者,但在Alpha多样性上无显著性差异(P>0.05);在菌门、纲、目分类水平上,不同基因型者肠道菌群相对丰度无显著性差异;但在�This research aims to explore the effects of high intensity interval training(HIIT)on gut microbiota and body composition of Han Chinese aged 18 to 24 years,and its association with the NPY gene rs16123 polymorphisms,with a view to providing individualized exercise and fitness guidance programs for people with different genetic characteristics by targeting gut microbiota. Methods:55 healthy Han College students were selected as subjects for HIIT intervention for 12 weeks. They were trained 3 times a week for 28 minutes each time,and the intensity of running and walking was 80—90% V·O_(2)max and 50—55% V·O_(2)max respectively. Their venous blood and feces were collected,and body composition was tested before intervention. Their feces were collected,and body composition were tested again after intervention. The Illumina HiSeq platform was used for analysis of gut microbiota after fecal DNA was extracted and amplified. Genotyping of NPY gene rs16123 polymorphism was completed by Illumina CGA gene chip. Results:(1)The distribution frequency of each genotype of NPY gene rs16123 polymorphism was consistent with HWE.(2)After 12 weeks of HIIT intervention,skeletal muscle mass,percentage of skeletal muscle mass,fat-free mass of all genotypes of NPY gene rs16123 polymorphism significantly increased,body fat percentage significantly decreased(P<0.05),and NPY gene rs16123 polymorphism was associated with the change rate of body fat percentage. That is,the decrease of body fat percentage in AA genotype was significantly higher than that in AG+GG genotype.(3)After 12 weeks of HIIT intervention,OTU numbers and richness of gut microbiota in AA genotype increased,and ACE index and Chao1 index in Alpha diversity increased significantly(P<0.05). Relative abundance of Gammaproteobacteria at the class level decreased significantly(P<0.05). Relative abundance of Enterobacteriales and Pasteurellales at the order level decreased significantly(P<0.05). Relative abundance of Enterobacteriaceae,Pasteurellaceae and Peptostreptococcace

关 键 词：肠道菌群 NPY 基因多态性 HIIT 体成分 

分 类 号：G804.2[文化科学—运动人体科学]