A Macroporous Cryogel with Enhanced Mechanical Properties for Osteochondral Regeneration In vivo  

作　　者：Xin-Yu Wu Jun Yang Fang-Hui Wu Wang-Bei Cao Tong Zhou Zhao-Yi Wang Chen-Xi Tu Zhong-Ru Gou Lei Zhang Chang-You Gao 

机构地区：[1]Ministry of Education,Key Laboratory of Macromolecular Synthesis and Functionalization,Department of Polymer Science and Engineering,Zhejiang University,Hangzhou,310027,China [2]Department of Orthopaedics,the Third Hospital Affiliated to Wenzhou Medical University&Rui’an People’s Hospital,Rui’an,325200,China [3]Bio-nanomaterials and Regenerative Medicine Research Division,Zhejiang-California International Nanosystem Institute,Zhejiang University,Hangzhou,310058,China

出　　处：《Chinese Journal of Polymer Science》2023年第1期40-50,共11页高分子科学（英文版）

基　　金：the Key research and development program of Zhejiang Province(No.2021C03113);the Natural Science Foundation of Zhejiang Province(No.LD21E030001).

摘　　要：The dense networks of conventional hydrogel hinder the efficient cell penetration and thus tissue regeneration.In this study,a macroporous cryogel with excellent mechanical properties was designed and fabricated through cryogelation and crosslinking of methacrylated gelatin(Gel-MA)and gelatin grafted with 2-ureido-4[1H]-6-methyl-pyrimidinone(Gel-UPY).The successful modification of the two functional units on the side chain of gelatin was confirmed by ^(1)H-NMR and infrared spectroscopy.The macroporous structure of cryogel was verified by scanning electron microscopy and confocal microscopy.The compressive modulus of cryogel(110 kPa)was 2 magnitudes higher than that of the conventional hydrogel of the same chemical compositions(8 kPa).Both the hydrogel and cryogel exhibited excellent stress-recovery ability.The cryogel could well maintain the viability of chondrocytes and facilitated their migration into the scaffold interior.After being implanted into osteochondral defects in rabbits for 12 w,the Gel-MA/Gel-UPY cryogel better facilitated the regeneration of upper cartilage and subchondral bone compared to the conventional hydrogel.

关 键 词：MACROPOROUS CRYOGEL Gelatin Ureido pyrimidinone Osteochondral regeneration 

分 类 号：O63[理学—高分子化学]