A Biosurfactant-containing TSD Strategy to Modify Bovine Pericardial Bioprosthetic Valves for Anticalcification  

作　　者：Cai-Yun Gao Gang Wang Lin Wang Qun-Song Wang Han-Cheng Wang Lin Yu Jian-Xiong Liu Jian-Dong Ding 

机构地区：[1]State Key Laboratory of Molecular Engineering of Polymers,Department of Macromolecular Science,Fudan University,Shanghai,200438,China [2]R&D Center,Lifetech Scientific(Shenzhen)Co.,Ltd.,Shenzhen,518057,China [3]R&D Center,Lifevalve Medical Scientific Co.,Ltd.,Shenzhen,518057,China

出　　处：《Chinese Journal of Polymer Science》2023年第1期51-66,共16页高分子科学（英文版）

基　　金：the National Natural Science Foundation of China(No.52130302).

摘　　要：Bioprosthetic heart valves(BHVs)are important for transcatheter valve replacement.Current commercial BHVs on the market are basically porcine or bovine pericardium(BP)crosslinked with glutaraldehyde(GA).Simply applying GA to BHVs can enhance mechanical stability,but cannot alleviate in vivo calcification.In this work,we developed a two-step decellularization(TSD)strategy to modify this biomacromolecular network,in which BP was post-treated,as the second step of decellularization,with a mild biosurfactant n-dodecyl-β-D-maltoside in a mixture of isopropanol and phosphate-buffered saline after the first step of traditional decellularization and GA cross-linking.The TSD-treated BP exhibited not only low cytotoxicity and excellent mechanical properties in vitro,but also low immune responses and significant anticalcification in vivo.After 60 days of subcutaneous implantation in the back of Wistar rats,the calcium content was,as quantified with an inductively coupled plasma optical emission spectrometer,only 1.1µg/mg compared to 138.6µg/mg in the control group without the post-treatment.In addition,collagen fibrils were observed with field emitting scanning electron microscopy(SEM),and the morphology and composition of the calcified sites resulting from in vivo biomineralization were studied with SEM with energy dispersive spectroscopy and also X-ray diffraction.This study proposes a facile yet effective anticalcification strategy for the modification of the bovine pericardial bioprosthetic heart valve,a natural biomacromolecular network.

关 键 词：Heart valve Anticalcification Collagen In vivo biomineralization Transcatheter aortic valve implantation(TAVI) Extracellular matrix Biomacromolecular network BIOSURFACTANT Bovine pericardium 

分 类 号：TQ333[化学工程—橡胶工业]