美花铁线莲茎乙醇提取物正丁醇萃取部位化学成分的提取和鉴定  

Chemical constituents from the stems of Clematis potaninii

作　　者：王啸洋 卫柯 陆云阳 佟菲[1] 张艳华[1] 汤海峰 WANG Xiaoyang;WEI Ke;LU Yunyang;TONG Fei;ZHANG Yanhua;TANG Haifeng(Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education),Department of Pharmacy,Peking University Cancer Hospital&Institute,Beijing 100142,China)

机构地区：[1]北京大学肿瘤医院、北京市肿瘤防治研究所药剂科/恶性肿瘤发病机制及转化研究教育部重点实验室,100142 [2]空军军医大学药学系

出　　处：《环球中医药》2022年第11期2010-2016,共7页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81274029);北京市属医院科研培育计划(PZ2021025);北京大学肿瘤医院科研基金(2021-26);北京大学肿瘤医院科创项目基金(KC2302)。

摘　　要：目的 研究美花铁线莲茎70%乙醇提取物正丁醇萃取部位的化学成分。方法 采用热回流法进行提取,对提取物的正丁醇萃取部位进行分离;运用多种分离手段包括正相硅胶、ODS反相硅胶、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等进行分离纯化;对单体化合物进行核磁共振和质谱检测,根据波谱数据(1D-NMR、2D-NMR及MS)和理化性质进行结构鉴定。结果 分离得到4个三萜类、3个木脂素类和1个甾醇苷类化合物,分别鉴定为木栓酮(1)、表木栓醇(2)、β-胡萝卜苷(3)、sargentodoside D (4)、落叶松脂醇-4,4’-二-O-β-D-吡喃葡萄糖苷(5)、落叶松脂醇-4-O-β-D-吡喃葡萄糖苷(6)、sieboldnoside A (7)和huzhangoside C (8)。结论 所有化合物均为首次从本植物中分离得到,化合物4为首次从铁线莲属植物中发现。Objective To study the chemical constituents from the n-butanol fraction of 70% ethanol extraction of the stems of Clematis potaninii.Methods Silica gel, reversed phase ODS, and Sephadex LH-20 gel column chromatographies and semi-preparation high performance liquid chromatography were used for separation and purification. The structures of the obtained compounds were identified by their spectral analysis(1D-NMR, 2D-NMR and MS) and physicochemical properties. Results Four triterpenoids, three lignans and one sterol glycoside were isolated and identified as friedelin(1), epifriedelinol(2), β-daucosterol(3), sargentodoside D(4), lariciresinol-4,4’-bis-O-β-D-glucopyranoside(5), lariciresinol-4-O-β-D-glucopyranoside(6), sieboldnoside A(7) and huzhangoside C(8), respectively. Conclusion All the compounds were isolated from C. potaninii for the first time, whlie compound 4 was the first time found from genus Clematis.

关 键 词：铁线莲属 美花铁线莲 三萜皂苷 木脂素 结构鉴定 分离纯化 

分 类 号：R284.1[医药卫生—中药学]