精浆同型半胱氨酸与精子形态、DNA碎片指数在男性不育患者中的相关性研究  

Correlation of seminal plasma homocysteine with sperm morphology and DNA fragmentation index in male infertility patients

作　　者：周莲[1] 符明昌[2] 蔡添娥 李娟[2] ZHOU Lian;FU Mingchang;CAI Tian′e;LI Juan(Department of Laboratory,Sanya Central Hospital(Hainan Third People′s Hospital),Sanya 572000,Hainan,China;Reproduction Laboratory,Sanya Central Hospital(Hainan Third People′s Hospital),Sanya 572000,Hainan,China)

机构地区：[1]三亚中心医院(海南省第三人民医院)检验科,海南三亚572000 [2]三亚中心医院(海南省第三人民医院)生殖实验室,海南三亚572000

出　　处：《中国性科学》2023年第1期23-26,共4页Chinese Journal of Human Sexuality

基　　金：2021年三亚市高校及医疗机构专项科技计划立项项目(2021GXYL07)。

摘　　要：目的研究精浆同型半胱氨酸(Hcy)与精子形态、DNA碎片指数(DFI)在男性不育患者中的相关性。方法选取2019年6月至2022年6月三亚中心医院(海南省第三人民医院)诊治的105例男性不育患者作为研究对象,设为疾病组。另选取同期116例进行体检的有生育史且身体健康的男性作为对照组。根据精液参数将男性不育患者分为正常型组(n=28)、少弱精子症组(n=33)、少畸精子症组(n=23)、畸弱精子症组(n=21)。根据精子DNA完整性将男性不育患者分为DFI≤15%组(n=20)、15%<DFI<30%组(n=64)、DFI≥30%组(n=21)。采用Pearson法分析精浆Hcy与精液参数、精子DFI的相关性。结果与对照组比较,疾病组精液体积、精子总数、前向运动精子率、正常形态精子率较低,精子DFI、精浆Hcy水平较高(P<0.05)。与正常型组比较,少弱精子症组、少畸精子症组、畸弱精子症组精浆Hcy水平较高(P<0.05);与少弱精子症组、少畸精子症组比较,畸弱精子症组精浆Hcy水平较高(P<0.05)。DFI≤15%组、15%<DFI<30%组、DFI≥30%组精浆Hcy水平依次升高(P<0.05)。Pearson法分析显示,男性不育患者精浆Hcy与前向运动精子率、正常形态精子率呈负相关(P<0.05);男性不育患者精浆Hcy与精子DFI呈正相关(P<0.05)。结论男性不育患者精浆Hcy呈高水平,其与前向运动精子率、正常形态精子率呈负相关性,与精子DFI呈正相关性。Objective To study the correlation between seminal plasma homocysteine(Hcy)and sperm morphology and DNA fragmentation index(DFI)in male infertility patients.Methods A total of 105 male infertile patients who were treated in Sanya Central Hospital(Hainan Third People′s Hospital)from June 2019 to June 2022 were collected as the disease group,and 116 healthy males with reproductive history who received physical examination were collected as the control group.According to the semen parameters,male infertility patients were separated into normal type group(n=28),oligospermia-asthenospermia group(n=33),oligospermia-teratozoospermia group(n=23)and asthenospermia-teratozoospermia group(n=21).According to the integrity of sperm DNA,male infertility patients were separated into DFI≤15%group(n=20),15%<DFI<30%group(n=64)and DFI≥30%group(n=21).The correlation of seminal plasma Hcy with semen parameters and sperm DFI was analyzed by Pearson method.Results Compared with the control group,the disease group had lower semen volume,total sperm count,rate of forward motile sperm and rate of normal morphological sperm(P<0.05),and higher levels of sperm DFI and seminal plasma Hcy(P<0.05).Compared with the normal group,the level of Hcy in seminal plasma of the oligospermia-asthenospermia group,the oligospermia-teratozoospermia group and the asthenospermia-teratozoospermia group were higher(P<0.05);compared with the oligospermia-asthenospermia group and the oligospermia-teratozaospermia group,the seminal plasma Hcy level of the asthenospermia-teratozoospermia group was higher(P<0.05).The level of seminal plasma Hcy in DFI≤15%group,15%<DFI<30%group and DFI≥30%group increased in turn(P<0.05).Pearson analysis showed that the seminal plasma Hcy of male infertility patients was negatively correlated with the rate of forward motility sperm and the rate of normal morphological sperm(P<0.05);seminal plasma Hcy was positively correlated with sperm DFI in male infertility patients(P<0.05).Conclusions The seminal plasma Hcy level in male

关 键 词：不育 同型半胱氨酸 精液参数 精子DNA碎片指数 相关性 

分 类 号：R697[医药卫生—泌尿科学]