一株耐盐碱细菌的筛选鉴定及其对植物的促生作用  

Isolation and identification of a saline-alkali resistant bacterium and its growth-promoting function on plant

作　　者：吕正阳 邵登科 张春源 赖泽成 李望豪 周瑞鹏 刘梓烨 王荣波 林占熺[1] 鲁国东[1,5] 叶文雨[1,2] LÜ Zhengyang;SHAO Dengke;ZHANG Chunyuan;LAI Zecheng;LI Wanghao;ZHOU Ruipeng;LIU Ziye;WANG Rongbo;LIN Zhanxi;LU Guodong;YE Wenyu(National Engineering Research Center of JUNCAO Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian University Key Laboratory for Plant￣Microbe Interaction,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Future Technology(Haixia Institute of Science and Technology),Fujian Agriculture and Forestry University,Fuzhou Fujian 350002,China;Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fuzhou,Fujian 350013,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002 [2]福建农林大学生命科学学院,植物与微生物相互作用福建省高校重点实验室,福建福州350002 [3]福建农林大学未来技术学院(海峡联合研究院),福建福州350002 [4]福建省作物有害生物监测与治理重点实验室,福建福州350013 [5]福建农林大学植物保护学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2023年第1期41-47,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建农林大学科技创新专项基金(KFA19137A、KFA19136A);福建省科技特派员项目(KTP21180A);福建省作物有害生物监测与治理重点实验室开放课题(MIMCP-202002);福建省科技重大专项(2021NZ029009);福建省引导性科技计划(2022N0010)。

摘　　要：采用平板稀释分离法、筛选培养基培养法和16S rRNA鉴定的方法,从福建莆田盐碱土壤中分离、筛选、鉴定得到1株耐盐碱能力最强的解淀粉芽孢杆菌(Bacillus amyloliquefaciens),命名为PT6-1.通过试验测定其生理生化特性和耐盐碱促生能力.结果表明,PT6-1多项生理生化特性呈阳性,在盐碱土培养条件下,施加PT6-1菌液对巨菌草株高、总鲜质量、总光合色素含量、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性相较于对照组分别增加了23.10%、45.73%、156.37%、68.14%、11.84%,对玉米各项指标分别增加了38.03%、123.02%、77.26%、18.25%、9.59%.PT6-1具有良好的耐盐碱促生能力,在盐碱地治理,菌草业、农业发展方面有良好的应用潜力.A strain of Bacillus amyloliquefaciens with the highest salt-alkali tolerance,named PT6-1,was isolated from salt-alkali soils in Putian City,Fujian Province by plate dilution separation,selection medium cultivation and 16S rRNA identification.The physiological and biochemical characteristics,and growth-promoting function on Cenchrus fungigraminus and maize were tested.The results showed that stain PT6-1 was capable of solubilizing inorganic phosphorus,fixing nitrogen and producing siderophore,and it also had certain growth-promoting potential.Application of PT6-1 aqueous solution was contributed to greater plant height,total fresh weight and total photosynthetic pigments content,higher peroxidase(POD)activity and catalase(CAT)activity of C.fungigraminus and maize,up by 23.10%,45.73%,156.37%,68.14%and 11.84%,respectively for C.fungigraminus,and up by 38.03%,123.02%,77.26%,18.25%and 9.59%for maize compared with the respective controls.PT6-1 had high saline-alkali tolerance and growth promotion ability,as well as great application potential for the improvement of saline-alkali soil,JUNCAO industry and agriculture.

关 键 词：耐盐碱 植物根际促生菌 巨菌草 玉米 促生作用 

分 类 号：Q93-331[生物学—微生物学]